2013年1月
Molecular Characterization of a Lipoxygenase from the Basidiomycete Mushroom Pleurotus ostreatus
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- ,
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- 巻
- 77
- 号
- 1
- 開始ページ
- 38
- 終了ページ
- 45
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.120484
- 出版者・発行元
- TAYLOR & FRANCIS LTD
The full-length cDNA of the gene PoLOX1 encoding a lipoxygenase (LOX) and its corresponding genomic DNA were isolated from the basidiomycete mushroom Pleurotus ostreatus strain H1. The deduced amino acid sequence of PoLOX1 showed similarity to a valencene dioxygenase of Pleurotus sapidus, putative LOX-like proteins from ascomycete, basidiomycete, and deuteromycete fungi, and known LOXs from plants, animals, and bacteria. PoLOX1 also contained the LOX iron-binding catalytic domain in the C-terminal region, but not the polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain, which is usually found in the N-terminal region of eukaryotic LOXs. Genomic sequence analysis revealed that PoLOX1 was interrupted by one intron, and that the promoter region included TATA and CAAT boxes. Southern blot analysis indicated that PoLOX1 is a member of a small gene family comprising highly similar genes. Northern blot analysis revealed that it is transcribed more abundantly in the stipes of the fruit bodies than in the caps.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.120484
- ISSN : 0916-8451
- eISSN : 1347-6947
- Web of Science ID : WOS:000316164800006