Jan, 2016
Novel process for producing 6-deoxy monosaccharides from l-fucose by coupling and sequential enzymatic method.
Journal of bioscience and bioengineering
- ,
- ,
- ,
- ,
- Volume
- 121
- Number
- 1
- First page
- 1
- Last page
- 6
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.jbiosc.2015.04.017
- Publisher
- SOC BIOSCIENCE BIOENGINEERING JAPAN
We biosynthesized 6-deoxy-L-talose, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose, which rarely exist in nature, from L-fucose by coupling and sequential enzymatic reactions. The first product, 6-deoxy-L-talose, was directly produced from L-fucose by the coupling reactions of immobilized D-arabinose isomerase and immobilized L-rhamnose isomerase. In one-pot reactions, the equilibrium ratio of L-fucose, L-fuculose, and 6-deoxy-L-talose was 80:9:11. In contrast, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose were produced from L-fucose by sequential enzymatic reactions. D-Arabinose isomerase converted L-fucose into L-fuculose with a ratio of 88:12. Purified L-fuculose was further epimerized into 6-deoxy-L-sorbose by D-allulose 3-epimerase with a ratio of 40:60. Finally, purified 6-deoxy-L-sorbose was isomerized into both 6-deoxy-L-gulose with an equilibrium ratio of 40:60 by L-ribose isomerase, and 6-deoxy-L-idose with an equilibrium ratio of 73:27 by D-glucose isomerase. Based on the amount of L-fucose used, the production yields of 6-deoxy-L-talose, 6-deoxy-L-sorbose, 6-deoxy-L-gulose, and 6-deoxy-L-idose were 7.1%, 14%, 2%, and 2.4%, respectively.
- Link information
- ID information
-
- DOI : 10.1016/j.jbiosc.2015.04.017
- ISSN : 1389-1723
- eISSN : 1347-4421
- Pubmed ID : 26031195
- Web of Science ID : WOS:000368744900001