論文

査読有り
2017年7月

Transcriptome Analysis of Skin from SMP30/GNL Knockout Mice Reveals the Effect of Ascorbic Acid Deficiency on Skin and Hair

IN VIVO
  • Koji Wakame
  • ,
  • Ken-Ichi Komatsu
  • ,
  • Akifumi Nakata
  • ,
  • Keisuke Sato
  • ,
  • Akira Takaguri
  • ,
  • Hirofumi Masutomi
  • ,
  • Takayuki Nagashima
  • ,
  • Hironobu Uchiyama

31
4
開始ページ
599
終了ページ
607
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.21873/invivo.11100
出版者・発行元
INT INST ANTICANCER RESEARCH

Background/Aim: Senescence marker protein30/gluconolactonase knockout mice (SMP-30/GNL-KO) are a very useful model for clarifying the involvement of vitamin C (VC) in aging-related diseases. In this study, the effects of VC deficiency on skin and hair growth were investigated using SMP-30/GNL-KO mice by RNA sequencing. Materials and Methods: SMP-30/GNL-KO mice were given water containing 1.5 g/l VC until up to 8 weeks after birth to maintain a VC concentration in their organs and plasma equivalent to that in wild-type mice. The mice were then divided into two groups: a VC(+) group, where VC was administered, and a VC(-) group, where VC was not administered. Skin samples were collected at 4 and 8 weeks after the treatment. RNA was extracted from each skin sample, followed by cDNA synthesis and RNA-seq. In addition, hair growth was compared between the VC(-) and VC(+) groups after shaving. Skin samples were collected from the shaved area for histological examination by hematoxylin & eosin (HE) staining. Results: RNA-seq revealed that there were 1,736 (FDR< 0.001) differentially expressed genes in the VC(-) and VC(+) groups. From the functional analysis of the differentially expressed genes in the VC(-) and VC(+) groups, predicted functionalities including cell death and cytotoxicity increased in the VC(+) group. Furthermore, it was predicted that the difference in hair growth between the VC(-) and VC(+) groups was caused by the expression of genes including keratin-related genes and the Sonic hedgehog gene. It was confirmed that hair growth was significantly promoted; hair growth from hair papilla cells was also confirmed by HE staining of the shaved backs of SMP-30/GNL-KO mice in the VC(+) group. Conclusion: RNA-seq of the skin from VCdeficient mice showed the effects of VC deficiency on the expression of genes involved in cell growth and the hair cycle. Visual inspection suggested that changes in the expression of the genes are involved in delaying hair growth in the VC(-) group. Further research on the relationship among VC deficiency, the hair cycle, and skin cell growth may contribute to research on hair restoration and skin aging.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.21873/invivo.11100
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28652426
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000404137100012&DestApp=WOS_CPL

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