論文

査読有り
2016年8月

Improved Proteome and Phosphoproteome Analysis on a Cation Exchanger by a Combined Acid and Salt Gradient

ANALYTICAL CHEMISTRY
  • Jun Adachi
  • ,
  • Kazunari Hashiguchi
  • ,
  • Maiko Nagano
  • ,
  • Misako Sato
  • ,
  • Ayako Sato
  • ,
  • Kazuna Fukamizu
  • ,
  • Yasushi Ishihama
  • ,
  • Takeshi Tomonaga

88
16
開始ページ
7899
終了ページ
7903
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.analchem.6b01232
出版者・発行元
AMER CHEMICAL SOC

Currently used elution methods for strong cation exchange (SCX) chromatography are based on two principles: salt arid pH gradient. In this paper, we report the first observation of peptide elution by acid gradient. The degree of peptide separation using C18-SCX StageTip was greatly improved hy our acid and salt-based elution method compared with a salt-based elution method. This development enabled us to identify over 22 000 phosphopeptides from 2 mg of protein without labor-intensive sample preparation. Our method is simple, robust, scalable, and low-cost and can be easily implemented without any special equipment or techniques.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.1021/acs.analchem.6b01232
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27436111
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000381654800006&DestApp=WOS_CPL

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