論文

査読有り
2012年1月

Mitochondria-Targeted Superoxide Dismutase (SOD2) Regulates Radiation Resistance and Radiation Stress Response in HeLa Cells

JOURNAL OF RADIATION RESEARCH
  • Ayaka Hosoki
  • ,
  • Shin-Ichiro Yonekura
  • ,
  • Qing-Li Zhao
  • ,
  • Zheng-Li Wei
  • ,
  • Ichiro Takasaki
  • ,
  • Yoshiaki Tabuchi
  • ,
  • Li-Li Wang
  • ,
  • Shiga Hasuike
  • ,
  • Takaharu Nomura
  • ,
  • Akira Tachibana
  • ,
  • Kazunari Hashiguchi
  • ,
  • Shuji Yonei
  • ,
  • Takashi Kondo
  • ,
  • Qiu-Mei Zhang-Akiyama

53
1
開始ページ
58
終了ページ
71
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1269/jrr.11034
出版者・発行元
OXFORD UNIV PRESS

Reactive oxygen species (ROS) act as a mediator of ionizing radiation-induced cellular damage. Previous studies have indicated that MnSOD (SOD2) plays a critical role in protection against ionizing radiation in mammalian cells. In this study, we constructed two types of stable He La cell lines overexpressing SOD2, He La S3/SOD2 and T-REx HeLa/SOD2, to elucidate the mechanisms underlying the protection against radiation by SOD2. SOD2 overexpression in mitochondria enhanced the survival of He La S3 and T-REx He La cells following gamma-irradiation. The levels of gamma H2AX significantly decreased in He La S3/SOD2 and T-REx HeLa/SOD2 cells compared with those in the control cells. MitoSox (TM) Red assays showed that both lines of SOD2-expressing cells showed suppression of the superoxide generation in mitochondria. Furthermore, flow cytometry with a fluorescent probe (2',7'-dichlorofluorescein) revealed that the cellular levels of ROS increased in He La S3 cells during post-irradiation incubation, but the increase was markedly attenuated in He La S3/SOD2 cells. DNA microarray analysis revealed that, of 47,000 probe sets analyzed, 117 and 166 probes showed more than 2-fold changes after 5.5 Gy of gamma-irradiation in control and HeLa S3/SOD2 cells, respectively. Pathway analysis revealed different expression profiles in irradiated control cells and irradiated SOD2-overexpressing cells. These results indicate that SOD2 protects HeLa cells against cellular effects of gamma-rays through suppressing oxidative stress in irradiated cells caused by ROS generated in the mitochondria and through regulating the expression of genes which play a critical role in protection against ionizing radiation.

Web of Science ® 被引用回数 : 62

リンク情報
DOI
https://doi.org/10.1269/jrr.11034
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22302046
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000300139200008&DestApp=WOS_CPL