1998年10月
Participation of cathepsins B and D in apoptosis of PC12 cells following serum deprivation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- 巻
- 251
- 号
- 1
- 開始ページ
- 199
- 終了ページ
- 203
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1006/bbrc.1998.9422
- 出版者・発行元
- ACADEMIC PRESS INC
Cathepsin D, a lysosomal aspartic proteinase, has been shown to induce apoptosis of HeLa cells when overexpressed. To further understand regulatory mechanisms of cathepsin D-induced cell death, we examined whether lysosomal cysteine and aspartic proteinases are involved in apoptosis of PC12 cells following serum deprivation. In serum deprived culture, PC12 cells overexpressing cathepsin D died more rapidly than wild-type cells. When the active forms of cathepsins B and D were examined during the apoptotic process of wild-type cells, the amount of cathepsin B was drastically reduced 24 hr after the onset of culture, whereas that of cathepsin D considerably increased. The viability of PC12 cells overexpressing cathepsin B was significantly higher in serum-deprived culture than wild-type cells. In this situation, the amount of the cathepsin B protein did not decrease. The results suggest that there exists an apoptotic pathway regulated by lysosomal cathepsins B and D. (C) 1998 Academic Press.
- リンク情報
- ID情報
-
- DOI : 10.1006/bbrc.1998.9422
- ISSN : 0006-291X
- PubMed ID : 9790930
- Web of Science ID : WOS:000076638800034