2013年1月
Perlecan regulates bidirectional Wnt signaling at the Drosophila neuromuscular junction
JOURNAL OF CELL BIOLOGY
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- 巻
- 200
- 号
- 2
- 開始ページ
- 219
- 終了ページ
- 233
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1083/jcb.201207036
- 出版者・発行元
- ROCKEFELLER UNIV PRESS
Heparan sulfate proteoglycans (HSPGs) play pivotal roles in the regulation of Wnt signaling activity in several tissues. At the Drosophila melanogaster neuromuscular junction (NMJ), Wnt/Wingless (Wg) regulates the formation of both pre- and postsynaptic structures; however, the mechanism balancing such bidirectional signaling remains elusive. In this paper, we demonstrate that mutations in the gene of a secreted HSPG, perlecan/trol, resulted in diverse postsynaptic defects and overproduction of synaptic boutons at NMJ. The postsynaptic defects, such as reduction in subsynaptic reticulum (SSR), were rescued by the postsynaptic activation of the Frizzled nuclear import Wg pathway. In contrast, overproduction of synaptic boutons was suppressed by the presynaptic down-regulation of the canonical Wg pathway. We also show that Trol was localized in the SSR and promoted postsynaptic accumulation of extracellular Wg proteins. These results suggest that Trol bidirectionally regulates both pre- and postsynaptic activities of Wg by precisely distributing Wg at the NMJ.
- リンク情報
- ID情報
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- DOI : 10.1083/jcb.201207036
- ISSN : 0021-9525
- PubMed ID : 23319599
- Web of Science ID : WOS:000313948100008