2005年8月
Inhibition of TRPC5 channels by Ca2+-binding protein 1 in Xenopus oocytes
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
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- 巻
- 450
- 号
- 5
- 開始ページ
- 345
- 終了ページ
- 354
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00424-005-1419-1
- 出版者・発行元
- SPRINGER
The transient receptor potential canonical type 5 (TRPC5) channel is a member of the channels that has been implicated in neurite extension and growth cone morphology of hippocampal neurons. Although homomeric TRPC5 channels are activated following stimulation of G(q/11)-coupled receptors, the exact mechanism for this activation remains unresolved. Using two-electrode voltage clamp recordings, we show that the activity of TRPC5 channels expressed in Xenopus oocytes is dependent on the presence of Ca2+ at the extracellular as well as the cytoplasmic side of the plasma membrane. TRPC5 was activated by the stimulation of coexpressed M5 muscarinic receptors or by ionomycin. The TRPC5 activity was detectable with the presence of submillimolar levels of extracellular Ca2+, but it was eliminated by the injection of 5 mM 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid into the oocytes. Lanthanum could substitute for extracellular Ca2+ to support TRPC5 activity. Coexpression of Ca2+-binding protein 1 (CaBP1), but not calmodulin (CaM), inhibited the TRPC5 activity, without affecting the cell surface expression of TRPC5 proteins. Using in vitro binding assays, we demonstrated direction interactions between CaBP1 and TRPC5. The CaBP1-binding sites at the C terminus of TRPC5 are closely localized, but not identical, to CaM-binding sites. We conclude that TRPC5 is a Ca2+-regulated channel, and its activity is negatively controlled by CaBP1.
- リンク情報
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- DOI
- https://doi.org/10.1007/s00424-005-1419-1
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000231313400008&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-23944500605&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0001-5152-5809
- ID情報
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- DOI : 10.1007/s00424-005-1419-1
- ISSN : 0031-6768
- ORCIDのPut Code : 25904579
- SCOPUS ID : 23944500605
- Web of Science ID : WOS:000231313400008