2006年4月
Aminoglutethimide prevents excitotoxic and ischemic injuries in cortical neurons
BRITISH JOURNAL OF PHARMACOLOGY
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- 巻
- 147
- 号
- 7
- 開始ページ
- 729
- 終了ページ
- 736
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/sj.bjp.0706636
- 出版者・発行元
- NATURE PUBLISHING GROUP
1 Aminoglutethimide is a clinically available drug that suppresses steroid biosynthesis by inhibiting enzymes such as cytochrome P450scc and aromatase. Because several members of neurosteroids regulate glutamate receptors, we investigated the effect of aminoglutethimide on cell death induced by overactivation of glutamate receptors in CNS neurons.
2 Long-term pretreatment of organotypic cerebrocortical slice cultures with aminoglutethimide ( 100 - 1000 mu M) for 6 days or over resulted in concentration-dependent suppression of neuronal cell death induced by NMDA. Aminoglutethimide ( 1000 mu M) also inhibited neurotoxicity of AMPA and kainate, but not of ionomycin or staurosporine.
3 The protective effect of aminoglutethimide against NMDA cytotoxicity was not mimicked by other steroid synthesis inhibitors including trilostane and exemestane, and was not reversed by concurrent application of steroids such as pregnenolone, estrone, 17 beta-estradiol and estriol.
4 In dissociated rat cerebrocortical cell cultures, long-term treatment with aminoglutethimide ( 10 1000 mu M) attenuated NMDA receptor-mediated glutamate cytotoxicity but produced no significant effect on glutamate-induced increases in intracellular Ca2+.
5 Brief as well as long-term pretreatment with aminoglutethimide ( 30 - 1000 mu M) prevented NMDA receptor-dependent ischemic neuronal injury in organotypic cerebrocortical slice cultures, which was associated with suppression of glutamate release during the ischemic insult.
6 These results indicate that aminoglutethimide, irrelevant to its actions on neurosteroid synthesis, protects CNS neurons from excitotoxic and ischemic injuries. Development of aminoglutethimide analogs possessing neuroprotective properties may be of therapeutic value.
2 Long-term pretreatment of organotypic cerebrocortical slice cultures with aminoglutethimide ( 100 - 1000 mu M) for 6 days or over resulted in concentration-dependent suppression of neuronal cell death induced by NMDA. Aminoglutethimide ( 1000 mu M) also inhibited neurotoxicity of AMPA and kainate, but not of ionomycin or staurosporine.
3 The protective effect of aminoglutethimide against NMDA cytotoxicity was not mimicked by other steroid synthesis inhibitors including trilostane and exemestane, and was not reversed by concurrent application of steroids such as pregnenolone, estrone, 17 beta-estradiol and estriol.
4 In dissociated rat cerebrocortical cell cultures, long-term treatment with aminoglutethimide ( 10 1000 mu M) attenuated NMDA receptor-mediated glutamate cytotoxicity but produced no significant effect on glutamate-induced increases in intracellular Ca2+.
5 Brief as well as long-term pretreatment with aminoglutethimide ( 30 - 1000 mu M) prevented NMDA receptor-dependent ischemic neuronal injury in organotypic cerebrocortical slice cultures, which was associated with suppression of glutamate release during the ischemic insult.
6 These results indicate that aminoglutethimide, irrelevant to its actions on neurosteroid synthesis, protects CNS neurons from excitotoxic and ischemic injuries. Development of aminoglutethimide analogs possessing neuroprotective properties may be of therapeutic value.
- リンク情報
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- DOI
- https://doi.org/10.1038/sj.bjp.0706636
- CiNii Articles
- http://ci.nii.ac.jp/naid/120002468468
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16474421
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000236702000005&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-33645534700&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0001-5152-5809
- ID情報
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- DOI : 10.1038/sj.bjp.0706636
- ISSN : 0007-1188
- CiNii Articles ID : 120002468468
- ORCIDのPut Code : 25904632
- PubMed ID : 16474421
- SCOPUS ID : 33645534700
- Web of Science ID : WOS:000236702000005