論文

査読有り
2013年6月

Rapid Structural Changes and Acidification of Guard Cell Vacuoles during Stomatal Closure Require Phosphatidylinositol 3,5-Bisphosphate

Plant Cell
  • Gwangbae Bak
  • ,
  • Eun-Jung Lee
  • ,
  • Yuree Lee
  • ,
  • Mariko Kato
  • ,
  • Shoji Segami
  • ,
  • Heven Sze
  • ,
  • Masayoshi Maeshima
  • ,
  • Jae-Ung Hwang
  • ,
  • Youngsook Lee

25
6
開始ページ
2202
終了ページ
2216
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1105/tpc.113.110411
出版者・発行元
AMER SOC PLANT BIOLOGISTS

Rapid stomatal closure is essential for water conservation in plants and is thus critical for survival under water deficiency. To close stomata rapidly, guard cells reduce their volume by converting a large central vacuole into a highly convoluted structure. However, the molecular mechanisms underlying this change are poorly understood. In this study, we used pH-indicator dyes to demonstrate that vacuolar convolution is accompanied by acidification of the vacuole in fava bean (Vicia faba) guard cells during abscisic acid (ABA)-induced stomatal closure. Vacuolar acidification is necessary for the rapid stomatal closure induced by ABA, since a double mutant of the vacuolar H+-ATPase vha-a2 vha-a3 and vacuolar H+-PPase mutant vhp1 showed delayed stomatal closure. Furthermore, we provide evidence for the critical role of phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P-2] in changes in pH and morphology of the vacuole. Single and double Arabidopsis thaliana null mutants of phosphatidylinositol 3-phosphate 5-kinases (PI3P5Ks) exhibited slow stomatal closure upon ABA treatment compared with the wild type. Moreover, an inhibitor of PI3P5K reduced vacuolar acidification and convolution and delayed stomatal closure in response to ABA. Taken together, these results suggest that rapid ABA-induced stomatal closure requires PtdIns(3,5)P-2, which is essential for vacuolar acidification and convolution.

Web of Science ® 被引用回数 : 69

リンク情報
DOI
https://doi.org/10.1105/tpc.113.110411
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000322371500024&DestApp=WOS_CPL
ID情報
  • DOI : 10.1105/tpc.113.110411
  • ISSN : 1040-4651
  • eISSN : 1532-298X
  • Web of Science ID : WOS:000322371500024

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