論文

査読有り
2016年5月

A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsis thaliana: stable association with plasma membrane and partial involvement in stomata closure

Journal of Plant Research
  • Chisako Nagata
  • ,
  • Chika Miwa
  • ,
  • Natsuki Tanaka
  • ,
  • Mariko Kato
  • ,
  • Momoe Suito
  • ,
  • Ayako Tsuchihira
  • ,
  • Yori Sato
  • ,
  • Shoji Segami
  • ,
  • Masayoshi Maeshima

129
3
開始ページ
539
終了ページ
550
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10265-016-0787-2
出版者・発行元
SPRINGER JAPAN KK

The Ca2+-binding protein-1 (PCaP1) of Arabidopsis thaliana is a new type protein that binds to phosphatidylinositol phosphates and Ca2+-calmodulin complex as well as free Ca2+. Although biochemical properties, such as binding to ligands and N-myristoylation, have been revealed, the intracellular localization, tissue and cell specificity, integrity of membrane association and physiological roles of PCaP1 are unknown. We investigated the tissue and intracellular distribution of PCaP1 by using transgenic lines expressing PCaP1 linked with a green fluorescence protein (GFP) at the carboxyl terminus of PCaP1. GFP fluorescence was obviously detected in most tissues including root, stem, leaf and flower. In these tissues, PCaP1-GFP signal was observed predominantly in the plasma membrane even under physiological stress conditions but not in other organelles. The fluorescence was detected in the cytosol when the 25-residue N-terminal sequence was deleted from PCaP1 indicating essential contribution of N-myristoylation to the plasma membrane anchoring. Fluorescence intensity of PCaP1-GFP in roots was slightly decreased in seedlings grown in medium supplemented with high concentrations of iron for 1 week and increased in those grown with copper. In stomatal guard cells, PCaP1-GFP was strictly, specifically localized to the plasma membrane at the epidermal-cell side but not at the pore side. A T-DNA insertion mutant line of PCaP1 did not show marked phenotype in a life cycle except for well growth under high CO2 conditions. However, stomata of the mutant line did not close entirely even in high osmolarity, which usually induces stomata closure. These results suggest that PCaP1 is involved in the stomatal movement, especially closure process, in leaves and response to excessive copper in root and leaf as a mineral nutrient as a physiological role.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1007/s10265-016-0787-2
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000375416300019&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s10265-016-0787-2
  • ISSN : 0918-9440
  • eISSN : 1618-0860
  • Web of Science ID : WOS:000375416300019

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