2018年5月1日
Sulfated vizantin induces formation of macrophage extracellular traps
Microbiology and Immunology
- 巻
- 62
- 号
- 5
- 開始ページ
- 310
- 終了ページ
- 316
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/1348-0421.12589
- 出版者・発行元
- Blackwell Publishing Asia
Vizantin is an insoluble adjuvant that activates macrophages and lymphocytes. Recently, 2,2′,3,3′,4,4′-hexasulfated-vizantin (sulfated vizantin), which enables solubilization of vizantin, was developed by the present team. Sulfated vizantin was found to enhance bactericidal activity against multi-drug resistant Pseudomonas aeruginosa in RAW264.7 cells. In addition, spread of P. aeruginosa was inhibited in RAW264.7 cells treated with sulfated vizantin. When only sulfated vizantin and P. aeruginosa were incubated, sulfated vizantin did not affect growth of P. aeruginosa. Formation of DNA-based extracellular traps (ETs), a novel defense mechanism in several types of innate immune cells, helps to eliminate pathogens. In the present study, ET-forming macrophages constituted the majority of immune cells. Sulfated vizantin induced ET formation in RAW264.7 cells, whereas a Ca-chelating reagent, EDTA, and T-type calcium channel blocker, tetrandrine, inhibited ET formation and attenuated inhibition of spread of P. aeruginosa in sulfated vizantin-treated cells. Thus, sulfated vizantin induces ET formation in phagocytic cells in a Ca-dependent manner, thus preventing spread of P. aeruginosa. Hence, sulfated vizantin may be useful in the management of infectious diseases.
- ID情報
-
- DOI : 10.1111/1348-0421.12589
- ISSN : 1348-0421
- ISSN : 0385-5600
- PubMed ID : 29577412
- SCOPUS ID : 85048044915