論文

査読有り
2014年

Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the growth plate cartilage of mice

Journal of Molecular Histology
  • Yasuhiko Bando
  • ,
  • Miyuki Yamamoto
  • ,
  • Koji Sakiyama
  • ,
  • Katsuyuki Inoue
  • ,
  • Shota Takizawa
  • ,
  • Yuji Owada
  • ,
  • Shoichi Iseki
  • ,
  • Hisatake Kondo
  • ,
  • Osamu Amano

45
5
開始ページ
507
終了ページ
518
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10735-014-9576-1
出版者・発行元
Kluwer Academic Publishers

n-3 Polyunsaturated fatty acids play a role in regulating the growth of the long bones. Fatty acid-binding proteins (FABPs) bind and transport hydrophobic long-chain fatty acids intracellularly, and epidermal-type FABP (E-FABP) has an affinity for n-3 fatty acids. This study aimed to clarify the localization of E-FABP in the growth plate of the mouse tibia. At the chondro-osseous junction (COJ) of the growth plate, E-FABP-immunoreactivity was exclusively localized in mononuclear, spindle-shaped cells with several long processes. These E-FABP-immunoreactive cells were identified as being septoclasts, i.e., cells that resorb uncalcified transverse septa. The processes of these immunoreactive septoclasts terminated between the longitudinal and transverse septa. E-FABP-immunoreactivity was found in the entire cytoplasm and on the mitochondrial outer membrane. In ontogeny, immunoreactive septoclasts were observed immediately after emergence of the primary ossifying center and were distributed not only at the COJ but also in the metaphysis near the COJ. The number of septoclasts increased at the postnatal age of 1 week (P1w)-P2w, and thereafter gradually decreased
and the cells became concentrated at the COJ after P3w-P4w. The immunoreactivity for peroxisome proliferator-activated receptor (PPAR)β/δ was detected in these E-FABP-immunoreactive septoclasts. The present results suggest that fatty acids, preferably n-3 ones, are intracellularly transported by E-FABP to various targets, including mitochondria and nucleus, in which PPARβ/δ may play functional roles in the transcriptional regulation of genes involved in the endochondral ossification. © 2014 Springer Science+Business Media.

リンク情報
DOI
https://doi.org/10.1007/s10735-014-9576-1
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24879443

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