We present here two cases of kidney transplantation from a non-heart beating deceased donor after long cold-ischemic time (CIT) more than 24 h. The donor was a young male who died of severe subarachnoid hemorrhage. Grafts were transplanted into two different patients one after another in our hospital. Their total ischemic times were 24 and 43 h because they were the fourth and fifth candidates on the waiting list. Although the anuric phase continued for more than three wk in both cases, finally their grafts functioned well with the nadir serum creatinine of 2.2 and 1.8 mg/dL, respectively. We evaluated protocol biopsies after 50 d from the transplantation. Both biopsy specimens revealed diffuse interstitial fibrosis, particularly more severe in the second recipient. The expression of 8-OHdG, one of the histochemical markers of oxidative stress, was more strongly expressed in the biopsy specimens of pre-perfusion and protocol biopsy in the same case. From these results, it was suspected that the interstitial fibrosis was induced by oxidative stress derived from the longer CIT and the following reperfusion.