論文

査読有り
2017年11月

Expression of cationic amino acid transporters in pig skeletal muscles during postnatal development

AMINO ACIDS
  • Aiko Ishida
  • ,
  • Akane Ashihara
  • ,
  • Kazuki Nakashima
  • ,
  • Masaya Katsumata

49
11
開始ページ
1805
終了ページ
1814
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00726-017-2478-2
出版者・発行元
SPRINGER WIEN

The cationic amino acid transporter (CAT) protein family transports lysine and arginine in cellular amino acid pools. We hypothesized that CAT expression changes in pig skeletal muscles during rapid pig postnatal development. We aimed to investigate the tissue distribution and changes in the ontogenic expression of CATs in pig skeletal muscles during postnatal development. Six piglets at 1, 12, 26, 45, and 75 days old were selected from six litters, and their longissimus dorsi (LD), biceps femoris (BF), and rhomboideus (RH) muscles, and their stomach, duodenum, jejunum, ileum, colon, liver, kidney, heart, and cerebrum were collected. CAT-1 was expressed in all the 12 tissues investigated. CAT-2 (CAT-2A isoform) expression was highest in the skeletal muscle and liver and lowest in the jejunum, ileum, kidney, and heart. CAT-3 was expressed mainly in the colon and detected in the jejunum, ileum, and cerebrum. The CAT-1 expression was higher in the skeletal muscle of day 1 pigs than in that of older pigs (P < 0.05). The CAT-2 mRNA level was lowest at day 1, but increased with postnatal development (P < 0.05). There was no significant change in CAT-1 expression among the LD, BF, and RH during postnatal development (P > 0.05); however, there was a change in CAT-2 expression. The CAT-2 expression was highest in the LD of 12-, 26-, 45-, and 75-day-old pigs, followed by the BF and RH (P < 0.05). These results suggest that CAT-1 and CAT-2 play different roles in pig skeletal muscles during postnatal development.


リンク情報
DOI
https://doi.org/10.1007/s00726-017-2478-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28803359
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000412914100002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00726-017-2478-2
  • ISSN : 0939-4451
  • eISSN : 1438-2199
  • PubMed ID : 28803359
  • Web of Science ID : WOS:000412914100002

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