論文

査読有り
2016年3月

Effect of dexamethasone on the expression of atrogin-1/MAFbx in chick skeletal muscle

ANIMAL SCIENCE JOURNAL
  • Kazuki Nakashima
  • ,
  • Aiko Ishida
  • ,
  • Daichi Ijiri
  • ,
  • Akira Ohtsuka

87
3
開始ページ
405
終了ページ
410
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/asj.12437
出版者・発行元
WILEY-BLACKWELL

Expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is high under catabolic conditions, that result in muscle atrophy. Messenger RNA (mRNA) expression of atrogin-1/MAFbx is increased by the glucocorticoid dexamethasone in mammalian skeletal muscle. This study investigated the effects of dexamethasone on expression of atrogin-1/MAFbx in skeletal muscle of neonatal chicks and in chick myotubes. Chicks were given a single intraperitoneal injection of dexamethasone at a concentration of 10mg/kg body weight. Twenty-four hours after dexamethasone administration, the Pectoralis muscle weight of chicks was decreased. mRNA expression of atrogin-1/MAFbx in skeletal muscle of chicks was significantly increased by dexamethasone administration. Expression of other proteolytic-related genes (20S proteasome C2 subunit, m-calpain large subunit, and cathepsin B) in skeletal muscle of chicks was not increased by dexamethasone administration. Chick myotubes were incubated with dexamethasone (1, 10 or 100 mu mol/L) for 6h. Expression of atrogin-1/MAFbx mRNA in chick myotubes was increased in the presence of all concentrations of dexamethasone. However, expression of other proteolytic-related genes (20S proteasome C2 subunit, m-calpain large subunit and cathepsin B) in chick myotubes was not affected by dexamethasone treatment. These results indicate that dexamethasone enhances atrogin-1/MAFbx expression in chick skeletal muscle, resulting in increased muscle atrophy.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1111/asj.12437
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26279163
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000372350900012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/asj.12437
  • ISSN : 1344-3941
  • eISSN : 1740-0929
  • PubMed ID : 26279163
  • Web of Science ID : WOS:000372350900012

エクスポート
BibTeX RIS