論文

査読有り
2017年7月

Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures

JOURNAL OF POULTRY SCIENCE
  • Kazuki Nakashima
  • ,
  • Aiko Ishida
  • ,
  • Saki Shimamoto
  • ,
  • Daichi Ijiri
  • ,
  • Akira Ohtsuka

54
3
開始ページ
247
終了ページ
252
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2141/jpsa.0160141
出版者・発行元
JAPAN POULTRY SCIENCE ASSOC

The expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is increased in catabolic conditions that result in muscle atrophy. The expression of atrogin-1/MAFbx mRNA is also decreased by the insulin-like growth factor-I (IGF-I) in mammalian skeletal muscle cell cultures. This study investigated the effect of IGF-I on the expression of atrogin-1/MAFbx in chicken skeletal muscle cell cultures. Chick myotubes were incubated with IGF-I for 1, 6, or 24 h. Protein content was increased by IGF-I (100 ng/ml) and incubated for 24 h in chick myotubes. The expression of atrogin-1/MAFbx mRNA decreased in the presence of IGF-I (1, 10, and 100 ng/ml) for 6h in chick myotubes. The expression of the m-calpain large subunit and cathepsin B mRNA was not decreased by IGF-I. Phosphorylation of Akt and FOXO1 increased in the presence of IGF-I (100 ng/ml) for 1 h in chick myotubes. These results indicate that IGF-I suppresses atrogin-1/MAFbx mRNA expression by phosphorylation of Akt and FOXO1, resulting in an increase in muscle growth in chick myotube cultures.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.2141/jpsa.0160141
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000406255200009&DestApp=WOS_CPL
ID情報
  • DOI : 10.2141/jpsa.0160141
  • ISSN : 1346-7395
  • Web of Science ID : WOS:000406255200009

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