2017年3月
Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
- 巻
- 4
- 号
- 開始ページ
- 115
- 終了ページ
- 125
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.omtm.2016.12.007
- 出版者・発行元
- CELL PRESS
Amyotrophic lateral sclerosis (ALS) is a progressive and fatal degenerative disorder of motor neurons (MNs). Embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs) now help us to understand the pathomechanisms of ALS via disease modeling. Various methods to differentiate ESCs/iPSCs into MNs by the addition of signaling molecules have been reported. However, classical methods require multiple steps, and newer simple methods using the transduction of transcription factors run the risk of genomic integration of the vector genes. Heterogeneity of the expression levels of the transcription factors also remains an issue. Here we describe a novel approach for differentiating human and mouse ESCs/iPSCs into MNs using a single Sendai virus vector encoding three transcription factors, LIM/homeobox protein 3, neurogenin 2, and islet-1, which are integration free. This single-vectormethod, generatingHB9-positive cells on day 2 from human iPSCs, increases the ratio of MNs to neurons compared to the use of three separate Sendai virus vectors. In addition, the MNs derived via this method from iPSCs of ALS patients and model mice display disease phenotypes. This simple approach significantly reduces the efforts required to generate MNs, and it provides a useful tool for disease modeling.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.omtm.2016.12.007
- ISSN : 2329-0501
- PubMed ID : 28344997
- Web of Science ID : WOS:000397454700011