2018年
Immunocytochemical Monitoring of PINK1/Parkin-Mediated Mitophagy in Cultured Cells.
Methods in molecular biology (Clifton, N.J.)
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- 巻
- 1759
- 号
- 開始ページ
- 19
- 終了ページ
- 27
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/7651_2017_20
Both PINK1 and parkin are the responsible genes (PARK6 and PARK2, respectively) for familial early-onset Parkinson's disease (PD). Several lines of evidences have suggested that mitochondrial dysfunction would be associated with PD pathogenesis. Lewy body, one of PD pathological hallmarks, contains alpha-synuclein, a familial PD (PARK1/4)-gene product, which is eliminated by macroautophagy, while PINK1 and parkin coordinately mediate mitophagy (hereafter called as PINK1/parkin-mediated mitophagy) reported firstly by Youle's group. The mitochondrial quality control system is specific for elimination of damaged mitochondria especially in the loss of mitochondrial membrane potential induced by treatment with mitochondrial uncoupler like CCCP or FCCP. In this chapter, we summarized immunocytochemical methods to monitor the PINK1/parkin-mediated mitophagy using cultured cells.
- ID情報
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- DOI : 10.1007/7651_2017_20
- ISSN : 1064-3745
- PubMed ID : 28361483