論文

査読有り 本文へのリンクあり 国際誌
2020年12月1日

Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets

Scientific Reports
  • Koichi Baba
  • Kei Sasaki
  • Mio Morita
  • Tomoyo Tanaka
  • Yosuke Teranishi
  • Takahiro Ogasawara
  • Yoshinori Oie
  • Izumi Kusumi
  • Masukazu Inoie
  • Ken ichiro Hata
  • Andrew J. Quantock
  • Masahiro Kino-oka
  • Kohji Nishida
  • 全て表示

10
1
開始ページ
9282
終了ページ
9282
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-64394-6

© 2020, The Author(s). Corneal limbal epithelial stem cell transplantation using cultivated human corneal epithelial cell sheets has been used successfully to treat limbal stem cell deficiencies. Here we report an investigation into the quality of cultivated human corneal epithelial cell sheets using time-lapse imaging of the cell culture process every 20 minutes over 14 days to ascertain the level of cell jamming, a phenomenon in which cells become smaller, more rounded and less actively expansive. In parallel, we also assessed the expression of p63, an important corneal epithelial stem cell marker. The occurrence of cell jamming was variable and transient, but was invariably associated with a thickening and stratification of the cell sheet. p63 was present in all expanding cell sheets in the first 9 days of culture, but it’s presence did not always correlate with stratification of the cell sheet. Nor did p63 expression necessarily persist in stratified cell sheets. An assessment of cell jamming, therefore, can shed significant light on the quality and regenerative potential of cultivated human corneal epithelial cell sheets.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-64394-6
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32518325
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283219
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85086157380&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85086157380&origin=inward
ID情報
  • DOI : 10.1038/s41598-020-64394-6
  • eISSN : 2045-2322
  • PubMed ID : 32518325
  • PubMed Central 記事ID : PMC7283219
  • SCOPUS ID : 85086157380

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