論文

査読有り
2021年3月

Development of a kinetic model expressing anomalous phenomena in human induced pluripotent stem cell culture

Journal of Bioscience and Bioengineering
  • Thi Nhu Trang Nguyen
  • ,
  • Kei Sasaki
  • ,
  • Masahiro Kino-oka

131
3
開始ページ
305
終了ページ
313
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiosc.2020.10.013
出版者・発行元
Elsevier BV

During culture with feeder cells, deviation from the undifferentiated state of human induced pluripotent stem cells (hiPSCs) occurs at a very low frequency. Anomalous cell migration in central and peripheral regions of hiPSC colonies has been suggested to be the trigger for this phenomenon. To confirm this hypothesis, sequential cell migration prior to deviation must be demonstrated. This has been difficult using in vitro methods. We therefore developed a kinetic model with a proposed definition of anomalous cell migration as continuous relatively fast or slow cell migration. The developed model was validated via in silico reproduction of deviation phenomenon observed in vitro, such as the positions of deviated cells in a colony and the frequency of deviation in culture. This model suggests that anomalous cell migration-driven hiPSC deviation can be explained by two factors: a mechanical stimulus, represented by cell migration, and duration of the mechanical stimulus. The factor "duration of mechanical stimulus" sets our model apart from others, and helps to realize the ultra-rare trigger (approximately 10-5) of deviation from the undifferentiated state in hiPSC culture.

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2020.10.013
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33262019
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85096866948&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85096866948&origin=inward
ID情報
  • DOI : 10.1016/j.jbiosc.2020.10.013
  • ISSN : 1389-1723
  • eISSN : 1347-4421
  • PubMed ID : 33262019
  • SCOPUS ID : 85096866948

エクスポート
BibTeX RIS