2017年3月
Increased expression of prion protein gene is accompanied by demethylation of CpG sites in a mouse embryonal carcinoma cell line, P19C6
JOURNAL OF VETERINARY MEDICAL SCIENCE
- ,
- ,
- ,
- ,
- 巻
- 79
- 号
- 3
- 開始ページ
- 644
- 終了ページ
- 648
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1292/jvms.16-0554
- 出版者・発行元
- JAPAN SOC VET SCI
Elucidation of the processes regulating the prion protein gene (Prnp) is an important key to understanding the development of prion disorders. In this study, we explored the involvement of DNA methylation in Prnp transcriptional regulation during neuronal differentiation of embryonic carcinoma P19C6 cells. When P19C6 cells were differentiated into neuronal cells, the expression of Prnp was markedly increased, while CpG methylation was significantly demethylated at the nucleotide region between-599 and-238 from the transcription start site. In addition, when P19C6 cells were applied in a DNA methyltransferase inhibitor, RG108, Prnp transcripts were also significantly increased in relation to the decreased methylation statuses. These findings helped to elucidate the DNA methylation-mediated regulation of Prnp expression during neuronal differentiation.
- リンク情報
- ID情報
-
- DOI : 10.1292/jvms.16-0554
- ISSN : 0916-7250
- eISSN : 1347-7439
- Web of Science ID : WOS:000397830400032