論文

査読有り
2003年10月

Analysis of T-cell receptor V beta gene from infiltrating T cells in insulitis and myocarditis in encephalomyocarditis virus-infected BALB/C mice

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • A Kawagishi
  • ,
  • A Kubosaki
  • ,
  • N Takeyama
  • ,
  • A Sakudo
  • ,
  • K Saeki
  • ,
  • Y Matsumoto
  • ,
  • T Hayashi
  • ,
  • T Onodera

310
3
開始ページ
791
終了ページ
795
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2003.09.078
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Encephalomyocarditis (EMC) virus induces insulin-dependent diabetes and myocarditis in several strains of mice. The T-cell receptor (TCR) Vbeta genes of infiltrating T cells in the pancreas and myocardium of BALB/C mice infected with EMC virus D-variant (EMC-D virus) were analyzed. Using a nested two-step polymerase chain reaction (PCR), TCR Vbeta cDNAs were cloned and sequenced. Two and four kinds of TCR Vbeta clones were obtained from T cells infiltrating into the pancreas and myocardium of BALB/ C mice infected with EMC-D virus, respectively. The infiltrating lymphocytes in the diabetic mice expressed Vbeta 8.1, 8.2, and 8.3 genes predominantly. Previously, the use of Vbeta 8.2 has been reported in autoimmune diseases such as murine experimental allergic encephalomyelitis (EAE) and non-obese diabetic (NOD) mouse. This study suggests that mice infected with EMC virus are a useful animal model for autoimmune diseases such as insulin-dependent diabetes. (C) 2003 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2003.09.078
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000185991400020&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2003.09.078
  • ISSN : 0006-291X
  • Web of Science ID : WOS:000185991400020

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