論文

査読有り
2012年8月

In situ Electrochemical-Transmission Surface Plasmon Resonance Spectroscopy for Poly(pyrrole-3-carboxylic acid) Thin-Film-Based Biosensor Applications

ACS APPLIED MATERIALS & INTERFACES
  • Rapiphun Janmanee
  • ,
  • Akira Baba
  • ,
  • Sukon Phanichphant
  • ,
  • Saengrawee Sriwichai
  • ,
  • Kazunari Shinbo
  • ,
  • Keizo Kato
  • ,
  • Futao Kaneko

4
8
開始ページ
4270
終了ページ
4275
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/am300970m
出版者・発行元
AMER CHEMICAL SOC

In this study, we describe the combination of transmission surface plasmon resonance (TSPR) an electrochemical techniques for the application to biosensors with conducting polymers. Electropolymerization was employed to construct poly(pyrrole-3-carboxylic acid) (PP3C) film on a gold coated grating substrate using pyrrole-3-carboxylic acid (P3C) monomer solution in 0.5 M H2SO4. In situ electrochemical transmission surface plasmon resonance (EC-TSPR), 1 measurements were carried out to study the kinetic and electroactivity properties of PP3C film. Immobilization of antihuman IgG on the activated surface and the binding process of human IgG and antihuman IgG in neutral solution could be detected in situ by EC-TSPR measurement. The surface modification steps on the PP3C layer led to an increase in intensity of the transmission peak. The performance, sensitivity, and utility of EC-TSPR spectroscopy showed obvious advantages for the detection of binding process with the simple experimental setup, and could be applied to the study of biomolecular interactions in various systems.

リンク情報
DOI
https://doi.org/10.1021/am300970m
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000307698600068&DestApp=WOS_CPL
ID情報
  • DOI : 10.1021/am300970m
  • ISSN : 1944-8244
  • Web of Science ID : WOS:000307698600068

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