2018年2月1日
Development of graphene oxide/poly(3,4-ethylenedioxythiophene)/poly(styrene sulfonate) thin film-based electrochemical surface plasmon resonance immunosensor for detection of human immunoglobulin G
Japanese Journal of Applied Physics
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- 巻
- 57
- 号
- 2
- 開始ページ
- 02CA07-1
- 終了ページ
- 02CA07-7
- 記述言語
- 英語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.7567/JJAP.57.02CA07
- 出版者・発行元
- Japan Society of Applied Physics
An electrochemically synthesized graphene oxide (GO)/poly(3,4-ethylenedioxythiophene) (PEDOT)/poly(styrene sulfonate) (PSS) thin film-based electrochemical surface plasmon resonance (EC-SPR) sensor chip was developed and employed for the detection of human immunoglobulin G (IgG). GO introduced the carboxylic group on the film surface, which also allowed electrochemical control, for the immobilization of the anti-IgG antibody via covalent bonding through amide coupling reaction. The SPR sensitivity of the detection was improved under the control by applying an electrochemical potential, by which the sensitivity was increased by the increment in applied potential. Among the open-circuit and different applied potentials in the range of -1.0 to 0.50 V, the EC-SPR immunosensor at an applied potential of 0.50 V exhibited the highest sensitivity of 6.08 × 10-3 mL μg-1 cm-2 and linearity in the human IgG concentration range of 1.0 to 10 μg mL-1 with a relatively low detection limit of 0.35 μg mL-1. The proposed sensor chip is promising for immunosensing at the physiological level.
- リンク情報
- ID情報
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- DOI : 10.7567/JJAP.57.02CA07
- ISSN : 1347-4065
- ISSN : 0021-4922
- eISSN : 1347-4065
- SCOPUS ID : 85040936426