Polystyrene sphere films were fabricated and the optical properties were investigated using surface plasmon spectroscopy (SPS) and emitted light due to reverse irradiation utilizing SPS Kretschmann configuration. The films were fabricated using sphere dispersed solution with various diameters from about 100 to 300 nm. The morphologies of the fabricated films were observed using atomic force microscopy and the sphere films had almost three layer structure. In SPS curve, large dip and shallow broad dip were observed at around 75° and 50°, respectively. The dips were considered to be due to the three layer structure and some defects in the film. Furthermore, emitted lights due to reverse irradiation were also observed as a function of the emitted angle in the Kretschmann configuration of the SPS measurement. Emitted lights due to surface plasmon excitation were also observed and the peak angle of the emitted light almost corresponded to the dip angle of the SPS curves.