Osakabe Keishi

J-GLOBAL         Last updated: Aug 27, 2019 at 01:08
 
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Name
Osakabe Keishi
Affiliation
The University of Tokushima
Research funding number
70450335
ORCID ID
0000-0002-6227-6913

Published Papers

 
An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice.
Toda E, Koiso N, Takebayashi A, Ichikawa M, Kiba T, Osakabe K, Osakabe Y, Sakakibara H, Kato N, Okamoto T
Nature Plants      Feb 2019   [Refereed]
OsMYB108 loss-of-function enriches p-coumaroylated and tricin lignin units in rice cell walls.
Miyamoto, T., Takada, R., Tobimatsu, Y., Takeda, Y., Suzuki, S., Yamamura, M., Osakabe, K., Osakabe, Y., Sakamoto, M., Umezawa, T.
Plant Journal      Feb 2019   [Refereed]
CRISPR/Cas9 mediated genome editing in Apple and Grapevine
Yuriko Osakabe, Liang Zhenchang, Ren Chong, Nishitani Chikako, Keishi Osakabe, Wada Masato, Komori Sadao, Malnoy Mickael, Velasco Riccardo, Poli Michele, Jung Min-Hee, Koo Ok-Jae, Viola Roberto, Kanchiswamy Nagamangala Chidananda
Nature Protocols   13 2844-2863   2018   [Refereed]
Lignin characterization of rice CONIFERALDEHYDE 5-HYDROXYLASE loss-of-function mutants generated with the CRISPR/Cas9 system. doi: 10.1111/tpj.14141.
Takeda Yuri, Suzuki Shiro, Tobimatsu Yuki, Keishi Osakabe, Yuriko Osakabe, Ragamustari Komara Safendrri, Sakamoto Masahiro, Umezawa Toshiaki
The Plant Journal : for Cell and Molecular Biology      Oct 2018   [Refereed]
Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. doi: 10.1371/journal.pone.0205117
Sugano S. Shigeo, Nishihama Ryuichi, Shirakawa Makoto, Takagi Junpei, Matsuda Yoriko, Ishida Sakiko, Shimada Tomoo, Hara-Nishimura Ikuko, Keishi Osakabe, Kohchi Takayuki
PLoS ONE   Vol.13(No.10) e0205117-e0205117   Oct 2018   [Refereed]
Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification. doi: 10.1111/tpj.13988
Takeda Yuri, Tobimatsu Yuki, Karlen D. Steven, Koshiba Taichi, Suzuki Shiro, Yamamura Masaomi, Murakami Shinya, Mukai Mai, Takefumi Hattori, Keishi Osakabe, Ralph John, Sakamoto Masahiro, Umezawa Toshiaki
The Plant Journal : for Cell and Molecular Biology      Jun 2018   [Refereed]
Hashimoto Ryosuke, Ueta Risa, Abe Chihiro, Yuriko Osakabe, Keishi Osakabe
Frontiers in Plant Science   doi:10.3389/fpls.2018.00916-doi:10.3389/fpls.2018.00916   Jun 2018   [Refereed]
Nakayasu Masaru, Akiyama Ryota, Lee Jae Hyoung, Keishi Osakabe, Yuriko Osakabe, Watanabe Bunta, Sugimoto Yukihiro, Umemoto Naoyuki, Saito Kazuki, Muranaka Toshiya, Mizutani Masaharu
Plant Physiology and Biochemistry : PPB   Vol.131 70-77   Apr 2018   [Refereed]
Potato (Solanum tuberosum) is a major food crop, while the most tissues of potato accumulates steroidal glycoalkaloids (SGAs) -solanine and -chaconine. Since SGAs confer a bitter taste on human and show the toxicity against various organisms, redu...
Sigeo Sugano, Hiroko Suzuki, Eisuke Shimokita, Hirofumi Chiba, Sumihare Noji, Yuriko Osakabe, Keishi Osakabe
Scientific Reports   Vol.7    Apr 2017   [Refereed]
Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cine...
Risa Ueta, Chihiro Abe, Ryosuke Ishihara, Takahito Watanabe, Sigeo Sugano, Hiroshi Ezura, Yuriko Osakabe, Keishi Osakabe
Scientific Reports   Vol.7 507-507   Mar 2017   [Refereed]
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the ...

Misc

 
中安大, LEE Hyoung Jae, 刑部敬史, 刑部祐里子, 杉本幸裕, 村中俊哉, 水谷正治
日本農芸化学会大会講演要旨集(Web)   2018 ROMBUNNO.2A27p07 (WEB ONLY)   Mar 2018

Books etc

 
“Crop Breeding Using CRISPR/Cas9”
Sugano, SS., Osakabe, K., Osakabe, Y*
“Crop Improvement Through Microbial Biotechnology” Elsevier   2018   
“Genome editing to improve abiotic stress responses in plants”
Osakabe, Y., Osakabe, K*
“Gene Editing in Plants” Progress in Molecular Biology and Translational Science” Elsevier   2017   
“Genome editing in higher plants”
Osakabe, Y., Osakabe, K*
“Targeted Genome Editing Using Engineered Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System” Springer -Verlag, Germany   2015   
“Plant environmental stress responses for survival and biomass enhancement” in “Climate change and abiotic stress tolerance”
Osakabe, Y.*, Osakabe, K., Shinozaki, K.
”Climate change and abiotic stress tolerance” Wiley-Blackwell, Wiley-VCH Verlag GmbH & Co., Germany.   2013   
"Abiotic stress responses in woody plants; molecular perspective in engineering woody plant tolerance to abiotic stress and enhance biomass"
Osakabe, Y.*, Kajita, S., and Osakabe, K.
Nova Science Publishers. USA   2012   

Conference Activities & Talks

 
Current and future of genome engineering in agricultural products [Invited]
Osakabe Keishi
Bioengineering of lignocellulose for clean energy production: perspectives and opportunities, Univ. Kyoto   27 Feb 2019   
Plant genome editing [Invited]
Keishi Osakabe
International Workshop of Plant Cell Wall Study   25 Oct 2018   Laigeng Li
Current and future of genome engineering for forest biotechnology [Invited]
Keishi Osakabe
International Symposium on Forest and Tree Molecular Biology and Biotechnology (FTMB2018)   24 Jul 2018   Vincent Chiang
“Current and future of genome editing in agricultural products” Plant Genome Editing & Genome Engineering, [Invited]
Keishi Osakabe
Vienna International Science Conferences and Events Association   3 Jul 2017   Vienna International Science Conferences and Events Association
Development of genome engineering for non-model and crop plants [Invited]
Keishi Osakabe
International Symposium on RNAi and Genome editing methods   16 Mar 2014   Sumihare Noji

Research Grants & Projects

 
「新奇ゲノム編集ツールの開発」
科学技術振興機構: 産学共創プラットフォーム共同研究推進プログラム(OPERA)研究領域「ゲノム編集による革新的な有用細胞・生物作成技術の創出」
Project Year: 2017 - 2021    Investigator(s): Osakabe Keishi
進化工学的および分子動力学的手法による新規ゲノム編集システムの創出
国立研究開発法人新エネルギー・産業技術総合開発機構: 植物の生産性制御に係る共通基盤技術開発
Project Year: 2016 - 2021    Investigator(s): Osakabe Keishi
Investigation of molecular mechanism for switching from vegetative growth to sexual growth in mushroom forming fungi
Grants-in-Aid for Scientific Research
Project Year: Apr 2017 - Mar 2020    Investigator(s): 坂本 裕一
Establishment of highly efficient chloroplast genome editing by using synthetic custom design nucleases
Grants-in-Aid for Scientific Research
Project Year: Apr 2013 - Mar 2016    Investigator(s): Osakabe Keishi