2010年11月
Crystallization and preliminary X-ray analysis of a marine diatom-infecting single-stranded RNA virus
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
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- 巻
- 66
- 号
- 開始ページ
- 1449
- 終了ページ
- 1452
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/S1744309110032422
- 出版者・発行元
- INT UNION CRYSTALLOGRAPHY
Crystals of a diatom-infecting virus (CtenRNAV) that diffracted to a resolution of 4.0 A were grown in a mixture of 2-methyl-2,4-pentanediol (MPD), calcium chloride and sodium acetate. It was possible to freeze the crystals directly at liquid-nitrogen temperature as the reservoir solution, which included about 30% MPD, acted as a cryoprotectant during X-ray diffraction data collection. A data set was collected from a single frozen crystal obtained using this method. The crystals belonged to space group P6(3)22, with unit-cell parameters a = b = 448.67, c = 309.76 A and two virus particles in the unit cell. The virus-particle orientation was determined using a rotation function and the virus-particle centre was estimated on the basis of crystallographic considerations. The packing of CtenRNAV in the crystal lattice was revealed by this preliminary crystallographic study.
- リンク情報
- ID情報
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- DOI : 10.1107/S1744309110032422
- ISSN : 1744-3091
- eISSN : 2053-230X
- Web of Science ID : WOS:000283714100010