2013年4月
Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 77
- 号
- 4
- 開始ページ
- 874
- 終了ページ
- 876
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.120936
- 出版者・発行元
- TAYLOR & FRANCIS LTD
A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 10(8) cells) is achieved.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.120936
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 23563551
- Web of Science ID : WOS:000319095500035