論文

査読有り 本文へのリンクあり
2019年12月1日

Major exopolysaccharide, EPS I, is associated with the feedback loop in the quorum sensing of Ralstonia solanacearum strain OE1-1

Molecular Plant Pathology
  • Kazusa Hayashi
  • ,
  • Wakana Senuma
  • ,
  • Kenji Kai
  • ,
  • Akinori Kiba
  • ,
  • Kouhei Ohnishi
  • ,
  • Yasufumi Hikichi

20
12
開始ページ
1740
終了ページ
1747
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/mpp.12870

The Gram-negative soil-borne bacterium Ralstonia solanacearum first infects roots of host plants and then invades xylem vessels. In xylem vessels, the bacteria grow vigorously and produce exopolysaccharides (EPSs) to cause a wilt symptom on host plants. The EPSs are thus the main virulence factors of R. solanacearum. The strain OE1-1 of R. solanacearum produces methyl 3-hydroxymyristate as a quorum-sensing (QS) signal, and senses this QS signal, activating QS. The QS-activated LysR-type transcriptional regulator PhcA induces the production of virulence-related metabolites including ralfuranone and the major EPS, EPS I. To elucidate the function of EPS I, the transcriptomes of R. solanacearum strains were analysed using RNA sequencing technology. The expression of 97.2% of the positively QS-regulated genes was down-regulated in the epsB-deleted mutant ΔepsB, which lost its EPS I productivity. Furthermore, expression of 98.0% of the negatively QS-regulated genes was up-regulated in ΔepsB. The deficiency to produce EPS I led to a significantly suppressed ralfuranone productivity and significantly enhanced swimming motility, which are suppressed by QS, but did not affect the expression levels of phcA and phcB, which encode a methyltransferase required for methyl 3-hydroxymyristate production. Overall, QS-dependently produced EPS I may be associated with the feedback loop of QS.

リンク情報
DOI
https://doi.org/10.1111/mpp.12870
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31560834
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85073939231&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85073939231&origin=inward
ID情報
  • DOI : 10.1111/mpp.12870
  • ISSN : 1464-6722
  • eISSN : 1364-3703
  • PubMed ID : 31560834
  • SCOPUS ID : 85073939231

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