論文

査読有り
2015年9月

WRKY Transcription Factors Phosphorylated by MAPK Regulate a Plant Immune NADPH Oxidase in Nicotiana benthamiana

PLANT CELL
  • Hiroaki Adachi
  • ,
  • Takaaki Nakano
  • ,
  • Noriko Miyagawa
  • ,
  • Nobuaki Ishihama
  • ,
  • Miki Yoshioka
  • ,
  • Yuri Katou
  • ,
  • Takashi Yaeno
  • ,
  • Ken Shirasu
  • ,
  • Hirofumi Yoshioka

27
9
開始ページ
2645
終了ページ
2663
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1105/tpc.15.00213
出版者・発行元
AMER SOC PLANT BIOLOGISTS

Pathogen attack sequentially confers pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) after sensing of pathogen patterns and effectors by plant immune receptors, respectively. Reactive oxygen species (ROS) play pivotal roles in PTI and ETI as signaling molecules. Nicotiana benthamiana RBOHB, an NADPH oxidase, is responsible for both the transient PTI ROS burst and the robust ETI ROS burst. Here, we show that RBOHB transactivation mediated by MAPK contributes to R3a/AVR3a-triggered ETI (AVR3a-ETI) ROS burst. RBOHB is markedly induced during the ETI and INF1-triggered PTI (INF1-PTI), but not flg22-tiggered PTI (flg22-PTI). We found that the RBOHB promoter contains a functional W-box in the R3a/AVR3a and INF1 signal-responsive cis-element. Ectopic expression of four phospho-mimicking mutants of WRKY transcription factors, which are MAPK substrates, induced RBOHB, and yeast one-hybrid analysis indicated that these mutants bind to the cis-element. Chromatin immunoprecipitation assays indicated direct binding of the WRKY to the cis-element in plants. Silencing of multiple WRKY genes compromised the upregulation of RBOHB, resulting in impairment of AVR3a-ETI and INF1-PTI ROS bursts, but not the flg22-PTI ROS burst. These results suggest that the MAPK-WRKY pathway is required for AVR3a-ETI and INF1-PTI ROS bursts by activation of RBOHB.

リンク情報
DOI
https://doi.org/10.1105/tpc.15.00213
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702210928615749
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365394100024&DestApp=WOS_CPL
URL
http://orcid.org/0000-0002-0349-3870
ID情報
  • DOI : 10.1105/tpc.15.00213
  • ISSN : 1040-4651
  • eISSN : 1532-298X
  • J-Global ID : 201702210928615749
  • ORCIDのPut Code : 23173143
  • Web of Science ID : WOS:000365394100024

エクスポート
BibTeX RIS