論文

査読有り
2011年1月

Distributed biological computation with multicellular engineered networks

NATURE
  • Sergi Rego
  • ,
  • Javier Macia
  • ,
  • Nuria Conde
  • ,
  • Kentaro Furukawa
  • ,
  • Jimmy Kjellen
  • ,
  • Tom Peeters
  • ,
  • Stefan Hohmann
  • ,
  • Eulalia de Nadal
  • ,
  • Francesc Posas*
  • ,
  • Ricard Sole*

469
7329
開始ページ
207
終了ページ
211
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/nature09679
出版者・発行元
NATURE PUBLISHING GROUP

Ongoing efforts within synthetic and systems biology have been directed towards the building of artificial computational devices(1) using engineered biological units as basic building blocks(2,3). Such efforts, inspired in the standard design of electronic circuits(4-7), are limited by the difficulties arising from wiring the basic computational units (logic gates) through the appropriate connections, each one to be implemented by a different molecule. Here, we show that there is a logically different form of implementing complex Boolean logic computations that reduces wiring constraints thanks to a redundant distribution of the desired output among engineered cells. A practical implementation is presented using a library of engineered yeast cells, which can be combined in multiple ways. Each construct defines a logic function and combining cells and their connections allow building more complex synthetic devices. As a proof of principle, we have implemented many logic functions by using just a few engineered cells. Of note, small modifications and combination of those cells allowed for implementing more complex circuits such as a multiplexer or a 1-bit adder with carry, showing the great potential for re-utilization of small parts of the circuit. Our results support the approach of using cellular consortia as an efficient way of engineering complex tasks not easily solvable using single-cell implementations.

リンク情報
DOI
https://doi.org/10.1038/nature09679
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21150900
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000286143400037&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/nature09679
  • ISSN : 0028-0836
  • PubMed ID : 21150900
  • Web of Science ID : WOS:000286143400037

エクスポート
BibTeX RIS