論文

査読有り 国際誌
2018年5月

Molecular pathogenesis of interstitial cystitis based on microRNA expression signature: miR-320 family-regulated molecular pathways and targets.

Journal of human genetics
  • Takayuki Arai
  • ,
  • Miki Fuse
  • ,
  • Yusuke Goto
  • ,
  • Kanya Kaga
  • ,
  • Akira Kurozumi
  • ,
  • Yasutaka Yamada
  • ,
  • Sho Sugawara
  • ,
  • Atsushi Okato
  • ,
  • Tomohiko Ichikawa
  • ,
  • Tomonori Yamanishi
  • ,
  • Naohiko Seki

63
5
開始ページ
543
終了ページ
554
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s10038-018-0419-x

Interstitial cystitis (IC), also known as bladder pain syndrome, is a chronic inflammatory disease that affects the bladder. The symptoms of IC vary, including feeling an urgent need for immediate urination and of needing to urinate often, as well as bladder or pelvic pain. Despite its high incidence, no molecular diagnostic methods are available for IC, and the molecular pathogenesis is unknown. microRNAs (miRNA) can regulate expression of RNA transcripts in cells and aberrant expression of miRNAs is associated with several human diseases. Here, we investigated the molecular pathogenesis of IC based on miRNA expression signatures. RNA sequencing of miRNA levels in IC tissues and comparison with levels in normal bladder tissue and bladder cancer revealed dysregulated expression of 366 miRNAs (203 and 163 down- and upregulated miRNAs, respectively). In particular, miR-320 family miRNAs(miR-320a, miR-320b, miR-320c, miR-320d and miR-320e) had downregulated expression in IC tissues. Genome-wide gene expression analyses and in silico database analyses showed that three transcription factors, E2F-1, E2F-2 and TUB, are regulated by miR-320 family miRNAs. Immunostaining of IC tissues confirmed that these transcription factors are overexpressed in IC tissues. Novel approaches that identify aberrantly expressed miRNA regulatory networks in IC could provide new prognostic markers and therapeutic targets for this disease.

リンク情報
DOI
https://doi.org/10.1038/s10038-018-0419-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29531336

エクスポート
BibTeX RIS