論文

査読有り 国際誌
2018年2月

Effects of adipokinetic hormone and its related peptide on maintaining hemolymph carbohydrate and lipid levels in the two-spotted cricket, Gryllus bimaculatus.

Bioscience, biotechnology, and biochemistry
  • Yi Jun Zhou
  • ,
  • Keisuke Fukumura
  • ,
  • Shinji Nagata

82
2
開始ページ
274
終了ページ
284
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2017.1422106

Adipokinetic hormone (AKH) regulates energy homeostasis in insects by mobilizing lipid and carbohydrate from the fat body. Here, using RNA sequencing data, we identified cDNAs encoding AKH (GbAKH) and its highly homologous hormone AKH/corazonin-related peptide (GbACP) in the corpora cardiaca of the two-spotted cricket, Gryllus bimaculatus. RT-PCR revealed that GbAKH and GbACP are predominantly expressed in the corpora cardiaca and corpora allata, respectively. Phylogenetic analysis confirmed that the identified GbAKH and GbACP belong to the clades containing other AKHs and ACPs, respectively. Injection of synthetic GbAKH and GbACP elevated hemolymph carbohydrate and lipid levels and reduced food intake significantly. In contrast, knockdown of GbAKH and GbACP by RNA interference increased the food intake, although hemolymph lipid level was not altered. Collectively, this study provides evidence that ACP regulates hemolymph carbohydrate and lipid levels in cricket, possibly collaborative contribution with AKH to the maintenance of energy homeostasis.

リンク情報
DOI
https://doi.org/10.1080/09168451.2017.1422106
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29325488
ID情報
  • DOI : 10.1080/09168451.2017.1422106
  • PubMed ID : 29325488

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