論文

査読有り
2016年3月1日

Enhancement of biodegradable plastic-degrading enzyme production from paraphoma-like fungus, strain B47-9

Journal of Oleo Science
  • Yuka Sameshima-Yamashita
  • ,
  • Motoo Koitabashi
  • ,
  • Wataru Tsuchiya
  • ,
  • Ken Suzuki
  • ,
  • Takashi Watanabe
  • ,
  • Yukiko Shinozaki
  • ,
  • Kimiko Yamamoto-Tamura
  • ,
  • Toshimasa Yamazaki
  • ,
  • Hiroko Kitamoto

65
3
開始ページ
257
終了ページ
262
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.5650/jos.ess15207
出版者・発行元
Japan Oil Chemists Society

To improve the productivity of Paraphoma-like fungal strain B47-9 for biodegradable plastic (BP)-degrading enzyme (PCLE), the optimal concentration of emulsified poly(butylene succinate-coadipate) (PBSA) in the medium was determined. Emulsified PBSA was consumed as a sole carbon source and an inducer of PCLE production by strain B47-9. Among the various concentrations of emulsified PBSA [0.09–0.9% (w/v)] used in flask cultivation, 0.27% yielded the maximum enzyme activity within a short cultivation period. To evaluate the residual concentration of emulsified PBSA in culture, emulsified PBSA in aliquots of culture supernatant was digested in vitro, and the concentration of released monomerised succinic acid was determined. Regardless of the initial concentration of emulsified PBSA in medium, PCLE activity was detected after residual succinic acid decreased below 0.04 mg/mL in culture broth. Jarfermentation was performed at a 0.27% PBSA concentration. Among the various airflow rates tested, 1 LPM resulted in a PCLE production rate of 1.0 U/mL/day. The enzyme activity in the resulting culture filtrate (4.2 U/2 mL) was shown to degrade commercial BP films (1 × 1 cm, 20 μm thickness) within 8 hours.

リンク情報
DOI
https://doi.org/10.5650/jos.ess15207
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26876678

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