論文

査読有り 国際誌
2020年5月

Fungal community dynamics during degradation of poly(butylene succinate-co-adipate) film in two cultivated soils in Japan.

Bioscience, biotechnology, and biochemistry
  • Kimiko Yamamoto-Tamura
  • ,
  • Yuko Takada Hoshino
  • ,
  • Shun Tsuboi
  • ,
  • Chuan Huang
  • ,
  • Ayaka Wenhong Kishimoto-Mo
  • ,
  • Yuka Sameshima-Yamashita
  • ,
  • Hiroko Kitamoto

84
5
開始ページ
1077
終了ページ
1087
記述言語
英語
掲載種別
DOI
10.1080/09168451.2020.1713718

Fungi play an important role in the degradation of biodegradable plastics (BPs) in soil. However, little is known about their dynamics in the soil during the degradation of BPs. We studied the community dynamics of BP-degrading fungi during poly(butylene succinate-co-adipate) (PBSA) film degradation in two different types of soils using culture-dependent and culture-independent methods. The Fluvisol and the Andosol soils degrade embedded PBSA films at high and low speeds, respectively. The number of PBSA emulsion-degrading fungi that increased in the Fluvisol soil was higher than that in the Andosol soil after embedding with PBSA films. We succeeded in detecting internal transcribed spacer 1 (ITS1) regions those matched that of the fungi by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) in both soils. Our results suggest that fungal community analyses using PCR-DGGE in combination with BP degraders isolation techniques enables the monitoring of BP films-degrading fungi.

リンク情報
DOI
https://doi.org/10.1080/09168451.2020.1713718
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31959072

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