Takayuki Ohnuma - My portal - researchmap

Profile Information

Affiliation: Associate professor, Faculty of Agriculture Department of Advanced Bioscience, Kindai University
: (Concurrent)

Degree: Ph. D.(Mar, 2002, Kyushu University)

J-GLOBAL ID: 201401074960673419
researchmap Member ID: B000235126

Research Interests

4

Research Areas

1

Life sciences / Applied biochemistry / 酵素学

Research History

7

2021 - Present

Faculty of Agriculture Department of Advanced Bioscience, Kindai University
2015 - 2021

Kinki University
2012 - 2015

Faculty of Agriculture, Kindai University
2008 - 2012

Faculty of Agriculture, Kindai University
2006 - 2008

National Institute of Agrobiological Sciences
2003 - 2006

University of California, Berkeley/USDA
2002 - 2003

博士研究員, 獣医学部, イリノイ大学アーバナ-シャンペーン校

Education

3

- Mar, 2002

Kyushu University
- Mar, 1999

Graduate School of Agriculture, Meijo University
- Mar, 1997

Faculty of Agriculture, Meijo University

Committee Memberships

5

Sep, 2021 - Present

「応用糖質科学」副編集委員長, 日本応用糖質科学会
2017 - Present

評議員, 日本応用糖質科学会
2014 - Present

編集委員, 日本キチン・キトサン学会
2013 - Present

和文誌編集員, 日本応用糖質科学会
2014 - 2016

理事, 日本キチン・キトサン学会

Awards

3

Sep, 2019

Technology Development Award, The Japanese Society of Applied Glycoscience
Aug, 2017

奨励賞, キチン分解酵素の構造と機能および糖鎖合成への応用, 日本キチン・キトサン学会

大沼貴之
Sep, 2015

prize for encouragement, Japanese Society of Applied Glycoscience

Takayuki Ohnuma

Papers

70

Synthesis of Oligoglucosamine Analogues Containing the N,N,N-Trimethyl-d-glucosaminyl Unit by Automated Electrochemical Assembly

Md Azadur RAHMAN, Shuji TAKAHASHI, Norihiko SASAKI, Toshiyuki ITOH, Takayuki OHNUMA, Toshiki NOKAMI

Electrochemistry, 91(11) 112013-112013, Nov 28, 2023
Periplasmic chitooligosaccharide-binding protein requires a three-domain organization for substrate translocation.

Takayuki Ohnuma, Jun Tsujii, Chikara Kataoka, Teruki Yoshimoto, Daijiro Takeshita, Outi Lampela, André H Juffer, Wipa Suginta, Tamo Fukamizo

Scientific reports, 13(1) 20558-20558, Nov 23, 2023
The first report of enzymatic transglycosylation catalyzed by family GH84 N-acetyl-β-d-glucosaminidase using a sugar oxazoline derivative as a glycosyl donor.

Tomonari Tanaka, Yoshiaki Habuchi, Rika Okuno, Shota Nishimura, Sotaro Tsuji, Yuji Aso, Takayuki Ohnuma

Carbohydrate research, 523 108740-108740, Jan 5, 2023
Characterization of two rice GH18 chitinases belonging to family 8 of plant pathogenesis-related proteins.

Jun Tanaka, Tomoya Takashima, Naojiro Abe, Tamo Fukamizo, Tomoyuki Numata, Takayuki Ohnuma

Plant science : an international journal of experimental plant biology, 111524-111524, Oct 31, 2022
Plant Chitinase Mutants as the Catalysts for Chitooligosaccharide Synthesis Using the Sugar Oxazoline Derivatives.

Naoyuki Umemoto, Natsuki Saito, Masato Noguchi, Shin-Ichiro Shoda, Takayuki Ohnuma, Takeshi Watanabe, Shohei Sakuda, Tamo Fukamizo

Journal of agricultural and food chemistry, 70(40) 12897-12906, Oct 12, 2022
Structure, mechanism, and phylogeny of LysM-chitinase conjugates specifically found in fern plants.

Yoshihito Kitaoku, Toki Taira, Tomoyuki Numata, Takayuki Ohnuma, Tamo Fukamizo

Plant science : an international journal of experimental plant biology, 321 111310-111310, Aug, 2022
A conserved loop structure of GH19 chitinases assists the enzyme function from behind the core-functional region.

Daiki Kawamoto, Tomoya Takashima, Tamo Fukamizo, Tomoyuki Numata, Takayuki Ohnuma

Glycobiology, 32(4) 356-364, Nov 22, 2021
Thermodynamic Analysis for Binding of 4-O-β-tri-N-acetylchitotriosyl Moranoline, a Transition State Analogue Inhibitor for Hen Egg White Lysozyme

Makoto Ogata, Tamo Fukamizo, Takayuki Ohnuma

Frontiers in Molecular Biosciences, 8 654706-654706, Jun 10, 2021
Chemo-enzymatic synthesis of Lacto-N-biose I catalyzed by β-1,3 galactosidase from Bacillus circulans using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside as a glycosyl donor.

Takayuki Ohnuma, Tomoki Taku, Takeshi Nagatani, Atsushi Horii, Shun Imaoka, Tomonari Tanaka

Bioscience, biotechnology, and biochemistry, 85(7) 1716-1719, Apr 20, 2021
Multi-functionality of a tryptophan residue conserved in substrate-binding groove of GH19 chitinases.

Takuya Nagata, Shoko Shinya, Takayuki Ohnuma, Tamo Fukamizo

Scientific reports, 11(1) 2494-2494, Jan 28, 2021 Peer-reviewed
Kinetic and thermodynamic insights into the inhibitory mechanism of TMG-chitotriomycin on Vibrio campbellii GH20 exo-β-N-acetylglucosaminidase.

Yusuke Morimoto, Shuji Takahashi, Yuta Isoda, Toshiki Nokami, Tamo Fukamizo, Wipa Suginta, Takayuki Ohnuma

Carbohydrate research, 499 108201-108201, Nov 20, 2020 Peer-reviewed
Crystal structure and biochemical characterization of CJP38, a β-1,3-glucanase and allergen of Cryptomeria japonica pollen.

Takashima T, Taku T, Yamanaka T, Fukamizo T, Numata T, Ohnuma T

Molecular immunology, 116 199-207, Nov, 2019 Peer-reviewed
Structures and chitin-binding properties of two N-terminal lysin motifs (LysMs) found in a chitinase from Volvox carteri.

Kitaoku Y, Nishimura S, Hirono T, Suginta W, Ohnuma T, Fukamizo T

Glycobiology, 29(7) 565-575, Jul, 2019 Peer-reviewed
Characterization of a GH Family 20 Exo-β-N-acetylhexosaminidase with Antifungal Activity from Streptomyces avermitilis.

Naoki Shirasaka, Koichi Harazono, Ryota Nakahigashi, Keigo Mitsui, Jun Tanaka, Sayaka Tanazawa, Masaru Mitsutomi, Takayuki Ohnuma

Journal of applied glycoscience, 66(3) 83-88, 2019 Peer-reviewed
A novel method for chemo-enzymatic synthesis of chitin oligosaccharide catalyzed by the mutant of inverting family GH19 chitinase using 4,6-dimethoxy-1,3,5-triazin-2-yl α-chitobioside as a glycosyl donor.

Ohnuma T, Tanaka T, Urasaki A, Dozen S, Fukamizo T

Journal of biochemistry, 165(6) 497-503, Dec, 2018 Peer-reviewed
Mode of action and specificity of a chitinase from unicellular microalgae, Euglena gracilis.

Feng Y, Kitaoku Y, Tanaka J, Taira T, Ohnuma T, Aachmann FL, Fukamizo T

Plant molecular biology, 97(6) 553-564, Aug, 2018 Peer-reviewed
NgcE^Sco Acts as a Lower-Affinity Binding Protein of an ABC Transporter for the Uptake of N,N'-Diacetylchitobiose in Streptomyces coelicolor A3(2).

Iinuma C, Saito A, Ohnuma T, Tenconi E, Rosu A, Colson S, Mizutani Y, Liu F, Świątek-Połatyńska M, Wezel GV …

Microbes and environments, 33(3) 272-281, Aug, 2018 Peer-reviewed
Structure and Enzymatic Properties of a Two-Domain Family GH19 Chitinase from Japanese Cedar (Cryptomeria japonica) Pollen

Tomoya Takashima, Tomoyuki Numata, Toki Taira, Tamo Fukamizo, Takayuki Ohnuma

Journal of Agricultural and Food Chemistry, 66(22) 5699-5706, Jun 6, 2018 Peer-reviewed
Unique GH18 chitinase from Euglena gracilis: full-length cDNA cloning and characterization of its catalytic domain.

Taira T, Gushiken C, Sugata K, Ohnuma T, Fukamizo T

Bioscience, biotechnology, and biochemistry, 82(7) 1-11, Apr, 2018 Peer-reviewed
NMR analysis of substrate binding to a two-domain chitinase: Comparison between soluble and insoluble chitins

Tomoya Takashima, Takayuki Ohnuma, Tamo Fukamizo

Carbohydrate Research, 458-459 52-59, Mar 22, 2018 Peer-reviewed

More

Misc.

15

Applications of Streptomyces Enzymes in Food Industry

10(1) 42-48, 2020
糖質加水分解酵素の機能改変による新規糖鎖合成法の開発

大沼貴之, 田中知成, 深溝慶

バイオサイエンスとインダストリー, 77(4) 312‐313, Jul 10, 2019
キチン質分解酵素の構造と機能および糖鎖合成への応用

大沼貴之

キチン・キトサン研究, 24(1) 38-44, Apr, 2018
Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes

Wade Abbott, Orly Alber, Ed Bayer, Jean-Guy Berrin, Alisdair Boraston, Harry Brumer, Ryszard Brzezinski, Anthony Clarke, Beatrice Cobucci-Ponzano, Darrell Cockburn …

GLYCOBIOLOGY, 28(1) 3-8, Jan, 2018
キチン質分解酵素の構造と機能および利用に関する研究

大沼貴之

応用糖質科学, 6(1) 15-20, Feb 20, 2016
等温滴定型カロリメトリーを用いた生体分子間相互作用解析

北奥喜仁, 大沼貴之

化学と生物, 53(12) 834-842, Nov 20, 2015
糖質関連酵素の最近の進歩‐2 リゾチーム遷移状態アナログの設計に基づく反応機構の検証

尾形慎, 碓氷泰市, 梅本尚之, 大沼貴之, 深溝慶, 沼田倫征

化学と生物, 52(12) 819-824, Dec 1, 2014
リゾチーム遷移状態アナログの合成と反応機構の解析

尾形慎, 碓氷泰市, 梅本尚之, 大沼貴之, 深溝慶, 沼田倫征

応用糖質科学, 4(4) 308-313, Nov, 2014 Peer-reviewedInvited
キチン質の酵素分解とオリゴ糖合成研究の新展開植物キチナーゼの構造と機能,その利用

大沼貴之, 平良東紀

応用糖質科学, 4(2) 121-126, May 20, 2014
Structure and function of a family GH19 chitinase, and its application for the synthesis of chitin oligosaccharides

OHNUMA Takayuki, DOZEN Satoshi, UMEMOTO Naoyuki, SHINYA Shoko, TAIRA Toki, NUMATA Tomoyuki, FUKAMIZO Tamo

18(2) 142-143, Jul 1, 2012
X-ray crystal structure analysis of lysozyme and new inhibitor : N-Acetyl chitooligosyl deoxynojirimycin (GN_n-M) complex

UMEMOTO Naoyuki, OGATA Makoto, SUZUKI Akari, OHNUMA Takayuki, NUMATA Tomoyuki, USUI Taichi, FUKAMIZO Tamo

18(2) 204-204, Jul 1, 2012
ノルウェイの森から

大沼貴之

応用糖質科学 : 日本応用糖質科学会誌 = Bulletin of applied glycoscience, 1(1), Jan 20, 2011
特異な糖質結合モジュールLysMドメインの構造と機能 : 溶菌やエリシター受容体など多様な機能をもつドメインとリガンドとの相互作用

大沼貴之, 翁長彰子, 深溝慶, 平良東紀

化学と生物, 47(10) 676-677, Oct 1, 2009
Advances in Analytical Methods for the Reaction Catalyzed by Glycosyl Hydrolases

FUKAMIZO Tamo, OHNUMA Takayuki

KAGAKU TO SEIBUTSU, 47(6) 404-411, Jun, 2009
Structure and Function of Family 50 Carbohydrate Binding Modules (LysM Domains) from Pteris ryukyuensis Chitinase-A

OHNUMA Takayuki, ONAGA Shoko, MURATA Katsuyoshi, FUKAMIZO Tamo, TAIRA Toki, KATOH Etsuko

Journal of applied glycoscience, 56(2) 97-104, Apr 20, 2009

Books and Other Publications

5

Lysozymes: Sources, Functions and Role in Diseas

Shoko Shinya, Takayuki Ohnuma, Ryszard Brzezinski, Romy K. Scheerle, Johanna Grassmann, Thomas Letzel, Tamo Fukamizo (Role: Contributor, Lysozyme Superfamily: Progress in Functional Analysis using ESI-MS and NMR Spectroscopy)

Nova Science Publishers, Inc., 2013 (ISBN: 9781622578429)
Marine Medicinal Glycomics

Naoyuki Umemoto, Takayuki Ohnuma, Tamo Fukamizo (Role: Contributor, Enzymatic Synthesis of Chitin Oligosaccharides with Longer Chains)

Nova Science Publishers, Inc., 2013 (ISBN: 9781626186491)
Functional Glyco-Materials:Their Development and Application to Foods II

(Role: Contributor)

2013 (ISBN: 9784781308234)
Protein and Peptide Analysis by LC-MS: Experimental Strategies (RSC Chromatography Monographs)

Thomas Letzel, Series Roger M Smith, Tamo Fukamizo, Takayuki Ohnuma, Seronei Chelulei Cheison, Christian Webhofer, Michael Schrader, Gabrielle Stohr, Andreas Tebbe, Boris Macek, Johanna Grassmann, Romy Scheerle, Michael Krappmann, Rene Wissiack, Friedrich Lottspeich, Johannes Hoos, Nicolas L Young, Benjamin A Garcia, Ulrich Kulozik, Wilfried M.A Niessen

Royal Society of Chemistry, Aug 8, 2011 (ISBN: 1849731829)
Chitin, Chitosan, Oligosaccharides and Their Derivatives: Biological Activities and Applications

(Role: Joint author)

CRC press, Jul, 2010

Research Projects

8

大腸菌特有の EvgS センサー活性化制御機構の解析

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2023 - Mar, 2026

江口陽子, 大沼貴之
新規酵素によるいもち病菌の植物免疫回避とイネのカウンター防御の分子機構の解明

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2022 - Mar, 2025

大沼貴之
イネXIPによる病原菌抵抗機構の分子基盤の解明

基盤研究(C), 日本学術振興会, Apr, 2019 - Mar, 2022

大沼貴之
植物の細胞壁構築に関与するキチナーゼ様タンパク質CTLの構造と機能

若手研究(B), 日本学術振興会, Apr, 2014 - Mar, 2016

大沼貴之
マメ科植物が生産するノッド因子加水分解酵素の構造と機能

研究奨励金, 公益財団法人農芸化学研究奨励会, Apr, 2014 - Mar, 2015

大沼貴之
植物のキチンオリゴ糖エリシター受容体タンパク質の構造と機能

若手研究(B), 日本学術振興会, Apr, 2011 - Mar, 2013

大沼貴之
Award of guest researcher scholarship

Norwegian University of Life Sciences, 2011

大沼貴之
等温滴定型熱量計によるLysMドメインとキチンオリゴ糖の相互作用機構の解明

特定国派遣研究者(ノルウェー生命科学大学), 日本学術振興会ーThe Research Council of Norway, Aug, 2010 - Sep, 2010

大沼貴之