Aug, 2003
Impairment of superoxide dismutase activation by N-terminally truncated prion protein (PrP) in PrP-deficient neuronal cell line
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- Volume
- 308
- Number
- 3
- First page
- 660
- Last page
- 667
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/S0006-29IX(03)01459-1
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Previous studies have reported a neuroprotective role for cellular prion protein (PrPC) against apoptosis induced by serum deprivation in an immortalized prion protein gene (Prnp)-deficient neuronal cell line, but the mechanisms remain unclear. In this study, to investigate the mechanisms by which PrPC prevents apoptosis, the authors compared apoptosis of Prnp(-/-) cells with that of Prnp(-/-) cells expressing the wild-type PrPC or PrPC lacking N-terminal octapeptide repeat region under serum-free conditions. Re-introduction of Prnp rescued cells from apoptosis, upregulated superoxide dismutase (SOD) activity, enhanced superoxide anion elimination, and inhibited caspase-3/9 activation. On the other hand, N-terminally truncated PrPC enhanced apoptosis accompanied by potentiation of superoxide production and caspase-3/9 activation due to inhibition of SOD. These results suggest that PrPC protects Prnp(-/-) cells from apoptosis via superoxide- and caspase-3/9-dependent pathways by upregulating SOD activity. Furthermore, the octapeptide repeat region of PrPC plays an essential role in regulating apoptosis and SOD activity. (C) 2003 Elsevier Inc. All rights reserved.
- Link information
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- DOI
- https://doi.org/10.1016/S0006-29IX(03)01459-1
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000184945400039&DestApp=WOS_CPL
- URL
- https://www.sciencedirect.com/science/article/pii/S0006291X03014591?via%3Dihub
- ID information
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- DOI : 10.1016/S0006-29IX(03)01459-1
- ISSN : 0006-291X
- Web of Science ID : WOS:000184945400039