2017年9月
分子医療に向けた安定で特異的なコイルドコイル構造形成(学内特別研究および国外研修)--(学内特別研究費報告書)
日本工業大学研究報告 = Report of researches, Nippon Institute of Technology
- ,
- 巻
- 47
- 号
- 2
- 開始ページ
- 71
- 終了ページ
- 74
- 記述言語
- 日本語
- 掲載種別
In the previous study, the artificial cationic coiled-coil protein, CCPC 140, was designed to have the structural frame of human skeletal muscle α-tropomyosin. This CCPC 140 showed superior cell-penetrating activity. Looking at thermal melting profiles of circular dichroism, although all the amino acid residues at the coiled-coil intramolecular interface in heptad repeat were conserved, the Tm of CCPC 140 was much higher than that of α-tropomyosin. This indicates that the residues at outer surface of coiled-coil motif can be a determinant of structure stability. In this study, I explored a novel mechanism for structure stabilization on coiled-coil proteins.
- リンク情報
- ID情報
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- ISSN : 2189-5449
- CiNii Articles ID : 120006350000