Keisuke Isobe

Profile Information

Affiliation: RIKEN Center for Advanced Photonics, RIKEN
: Kyoto University

J-GLOBAL ID: 201401069200604405
researchmap Member ID: B000242641

External link: http://kaken.nii.ac.jp/d/r/10462657.ja.html

Research Interests

Research Areas

Nanotechnology/Materials / Optical engineering and photonics /

Research History

Apr, 2013 - Present

RIKEN
Apr, 2012 - Mar, 2013

RIKEN
Apr, 2011 - Mar, 2012

RIKEN
Apr, 2008 - Mar, 2011

RIKEN
Apr, 2007 - Mar, 2008

RIKEN

Awards

Feb, 2013

Research Award, Research Foundation for Opto-Science and Technology

Keisuke Isobe
May, 2012

Best Presentation Award, The Laser Society of Japan

Keisuke Isobe
Mar, 2012

The Konica Minolta Imaging Science Award, the Konica Minolta Science and Technology Foundation

Keisuke Isobe
Oct, 2011

Poster Award

Keisuke Isobe
Mar, 2011

The 2nd Research Incentive Award, Study on nonlinear optical microspectroscopy employing ultrabroadband laser pulses, RIKEN

Keisuke Isobe
Jan, 2011

6. Poster Award, Engineering Prize, at 2010 SPDR & FPR Presentation of Research Results, Stimulated parametric emission microspectroscopy, RIKEN

Keisuke Isobe
Sep, 2008

Young Scientist Oral Presentation Award, The Japan Society of Applied Physics

Keisuke Isobe

Papers

多焦点面を同時観察するための多光子蛍光顕微鏡と多光子パターン照明

磯部圭佑

フォトニクスニュース, 8 157-161, Mar 10, 2023
Fast z-focus controlling and multiplexing strategies for multiplane two-photon imaging of neural dynamics.

Kei N Ito, Keisuke Isobe, Fumitaka Osakada

Neuroscience research, Mar 31, 2022
Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing

Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Takayuki Michikawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Applied Physics Express, 15(4) 042005-1-042005-5, Mar 28, 2022 Peer-reviewedCorresponding author
Adaptive optics with spatio-temporal lock-in detection for temporal focusing microscopy

Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Optics Express, 29(18) 29021-29021, Aug 30, 2021 Peer-reviewedCorresponding author
Enhancement of optical sectioning capability of temporal focusing microscopy by using time-multiplexed multi-line focusing

Kenta Inazawa, Keisuke Isobe, Tomohiro Ishikawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Applied Physics Express, 14(8) 082008-082008, Aug 1, 2021 Peer-reviewedCorresponding author
Temporally multiplexed dual-plane imaging of neural activity with four-dimensional precision.

Masanari Onda, Ryosuke F Takeuchi, Keisuke Isobe, Toshiaki Suzuki, Yuji Masaki, Nao Morimoto, Fumitaka Osakada

Neuroscience research, 171 9-18, Feb 16, 2021 Peer-reviewed
Resistance to optical distortions in three-dimensional interferometric temporal focusing microscopy

Qiyuan Song, Keisuke Isobe, Katsumi Midorikawa, Fumihiko Kannari

OPTICS COMMUNICATIONS, 430 486-496, Jan, 2019 Peer-reviewed
Interferometric temporal focusing microscopy using three-photon excitation fluorescence.

Toda K, Isobe K, Namiki K, Kawano H, Miyawaki A, Midorikawa K

Biomedical optics express, 9(4) 1510-1519, Apr, 2018 Peer-reviewedCorresponding author
Temporal focusing microscopy using three-photon excitation fluorescence with a 92-fs Yb-fiber chirped pulse amplifier

Keisuke Toda, Keisuke Isobe, Kana Namiki, Hiroyuki Kawano, Atsushi Miyawaki, Katsumi Midorikawa

BIOMEDICAL OPTICS EXPRESS, 8(6) 2796-2806, Jun, 2017 Peer-reviewedCorresponding author
Temporal focusing microscopy combined with three-dimensional structured illumination

Keisuke Isobe, Keisuke Toda, Qiyuan Song, Fumihiko Kannari, Hiroyuki Kawano, Atsushi Miyawaki, Katsumi Midorikawa

JAPANESE JOURNAL OF APPLIED PHYSICS, 56(5), May, 2017 Peer-reviewed
Multiphoton 3D structured illumination microscopy for enhanced axial resolution in deep imaging

Qiyuan Song, Keisuke Isobe, Fumihiko Kannari, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, Katsumi Midorikawa

2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015, 3, Jan 7, 2016 Peer-reviewed
Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy

Qiyuan Song, Aoi Nakamura, Kenichi Hirosawa, Keisuke Isobe, Katsumi Midorikawa, Fumihiko Kannari

REVIEW OF SCIENTIFIC INSTRUMENTS, 86(8), Aug, 2015 Peer-reviewed
Spatial Overlap Modulation Nonlinear Optical Microscopy for Background-Free Deep Imaging

Isobe Keisuke, Midorikawa Katsumi

Nippon Laser Igakkaishi, 36(2) 210-215, 2015
2D simultaneous spatial and temporal focusing multiphoton microscopy for fast volume imaging with improved sectioning ability

Qiyuan Song, Keisuke Isobe, Kenichi Hirosawa, Katsumi Midorikawa, Fumihiko Kannari

Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 9329, 2015 Peer-reviewed
2D simultaneous spatial and temporal focusing multiphoton microscopy for fast volume imaging with improved sectioning ability

Qiyuan Song, Keisuke Isobe, Kenichi Hirosawa, Katsumi Midorikawa, Fumihiko Kannari

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XV, 9329, 2015 Peer-reviewed
Temporal focusing microscopy with structured illumination for super-resolution deep imaging

Keisuke Isobe, Kyohei Mochizuki, Qiyuan Song, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, Katsumi Midorikawa

2014 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), 2014 Peer-reviewed
Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination

Keisuke Isobe, Takanori Takeda, Kyohei Mochizuki, Qiyuan Song, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, Katsumi Midorikawa

BIOMEDICAL OPTICS EXPRESS, 4(11) 2396-2410, Nov, 2013 Peer-reviewedLead authorCorresponding author
Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector

Keisuke Isobe, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, Katsumi Midorikawa

BIOMEDICAL OPTICS EXPRESS, 4(10) 1937-1945, Oct, 2013 Peer-reviewedLead authorCorresponding author
Simultaneous imaging of two-photon absorption and stimulated raman scattering by spatial overlap modulation nonlinear optical microscopy

Keisuke Isobe, Hiroyuki Kawano, Akira Suda, Atsushi Miyawaki, Atsushi Miyawaki, Katsumi Midorikawa

Biomedical Optics Express, 4(9) 1548-1558, Sep 1, 2013 Peer-reviewedLead authorCorresponding author
Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy

Keisuke Isobe, Hiroyuki Kawano, Takanori Takeda, Akira Suda, Akiko Kumagai, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

BIOMEDICAL OPTICS EXPRESS, 3(7) 1594-1608, Jul, 2012 Peer-reviewedLead authorCorresponding author

Misc.

Super-Resolution Deep Imaging by Spatio-Temporal Control of Excitation Pulses

44(10) 653-657, Oct, 2016
Deep imaging by spatial overlap modulation nonlinear optical microscopy

35(8) 74-78, Aug, 2016
非線形光学イメージング技術の発展

磯部圭佑

分光研究 = Journal of the spectroscopical research of Japan, 62(5) 235-237, Oct 15, 2013
Nobel nonlinear optical microscopy for super-resolution deep imaging

ISOBE K., KAWANO H., TAKEDA T., SUDA A., KUMAGAI A., MIYAWAKI A., MIDORIKAWA K.

421 13-17, Dec 19, 2011
Measurement of two-photon excitation spectra of fluorescent proteins with Fourier-transform nonlinear spectroscopy

SUDA A., ISOBE K., HASHIMOTO H., KANNARI F., KAWANO H., MIZUNO H., MIYAWAKI A., MIDORIKAWA K.

404 49-53, Sep 13, 2010
Nonlinear optical microscopy employing spectral phase modulation

ISOBE Keisuke, SUDA Akira, KAWANO Hiroyuki, MIZUNO Hideaki, MIYAWAKI Atsushi, HASHIMOTO Hiroshi, KANNARI Fumihiko, MIDORIKAWA Katsumi

2009(1) 25-28, Feb 13, 2009
1H16-4 Subcellular dynamics analysis of proteins by two-photon FRAP

HIGASHI Tsunehito, ISOBE Keisuke, WATANABE Wataru, MATSUNAGA Sachhiro, ITOH Kazuyoshi, FUKUI Kiichi

17 217-217, 2005
Analyses of fluorescence proteins by multiple-wavelength excitation fluorescence microscopy using supercontinuum

Kiichi Fukui, Sachihiro Mastunaga, Keisuke Isobe, Tsunehito Higashi, Wataru Watanabe, Kazuyoshi Itoh

CELL STRUCTURE AND FUNCTION, 29 59-59, May, 2004
3S2-AM2 Visualiztion of bio-molecules in living cells by laser 3D microscopy

FUKUI Kiichi, MATSUNAGA Sachihiro, HIGASHI Tsunehito, ISOBE Keisuke, WATANABE Wataru, ITOH Kazuyoshi

16 40-40, 2004

Presentations

Scanless two-photon patterned illumination with high axial resolution

Kenta Inazawa, Keisuke Isobe, Takayuki Michikawa, Kana Namiki, Atsushi Miyawaki, Katsumi Midorikawa

The 12th Asia-Pacific Laser Symposium, Sep 5, 2023
Large-scale simultaneous measurements of mouse cerebral and cerebellar activity during voluntary movements

T. Michikawa, K. Isobe, A. Miyawaki, K. Midorikawa

Dec 20, 2022
随意運動中のマウス大脳および小脳活動のカルシウムイメージングによる大規模同時計測

道川貴章, 磯部圭佑, 宮脇敦史, 緑川克美

第 9 回日本 BMI 研究会, Oct 22, 2022
時間多重化時空間集光を用いた３次元多光子パターン照明技術

磯部圭佑, 道川貴章, 並木香奈, 宮脇敦史, 緑川克美

第83回応用物理学会秋季講演会, Sep 21, 2022
Adaptive optics for temporal focusing and two-photon patterned illumination

K. Isobe

International;Symposium on Comprehensive understanding of scattering;fluctuated fields;science of clairvoyance, Apr 22, 2022 Invited
Fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed temporal focusing pulse

K. Isobe, T. Ishikawa, K. Inazawa, T. Michikawa, F. Kannari, K. Midorikawa

The 11th Advanced Lasers and Photon Sources, Apr 18, 2022
超短光パルスの時空間制御による多光子イメージングと光操作

磯部圭佑, 緑川克美

レーザー学会学術講演会第42回年次大会, Jan 14, 2022
大規模カルシウムイメージングによるマウス大脳および小脳における感覚情報表現の解析

道川貴章, 磯部圭佑, 宮脇敦史, 緑川克美

第 8 回日本 BMI 研究会, Nov 6, 2021
多平面同時多光子イメージングと多光子パターン照明

磯部圭佑

レーザー顕微鏡研究会, Nov 5, 2021 Invited
Large-Scale Optical Imaging of Neural Activity in the Mouse Cerebrum and Cerebellum

T. Michikawa, K. Isobe, A. Miyawaki, K. Midorikawa

EMBO | EMBL Symposium, Seeing is Believing: Imaging the Molecular Processes of Life, Oct 6, 2021
Background suppression of structured illumination microscopy by using time-multiplexed multi-line temporal-focusing

K. Isobe, K. Inazawa, T. Ishikawa, K. Namiki, A. Miyawaki, F. Kannari, K. Midorikawa

EMBO | EMBL Symposium, Seeing is Believing: Imaging the Molecular Processes of Life, Oct 5, 2021
多焦点面同時多光子イメージング技術の開発

磯部圭佑, 恩田将成, 竹内遼介, 伊藤慶, 佐藤彰典, 道川貴章, 宮脇敦史, 小坂田文隆, 緑川克美

第82回応用物理学会秋季講演会, Sep 21, 2021
超短光パルスの時空間分布制御による多光子イメージングと光操作技術

磯部圭佑

強光子場科学研究懇談会, Jul 9, 2021 Invited
Enhancement of axial resolution of temporal focusing microscopy by using programmable time-multiplexed multi-line focusing

K. Isobe, K. Inazawa, T. Ishikawa, F. Kannari, K. Namiki, A. Miyawaki, K. Midorikawa

The 10th Advanced Lasers and Photon Sources, Apr 21, 2021
Adaptive optics of wide-field temporal focusing microscopy with structured illumination microscopy

T. Ishikawa, K. Isobe, K. Inazawa, F. Kannari, K. Midorikawa

The 10th Advanced Lasers and Photon Sources, Apr 21, 2021
Transient dynamics of an ANDi mode-locked Yb-fiber laser oscillator monitored by time-stretch dispersive Fourier transform

T. Ishikawa, K. Isobe, M. Yoshizawa, F. Kannari, K. Midorikawa

The 10th Advanced Lasers and Photon Sources, Apr 19, 2021

Industrial Property Rights

特許第5137026号 2光子励起蛍光観察方法及び装置

磯部圭佑, 須田亮, 緑川克美, 神成文彦, 宮脇敦史
WO2012-127907 非線形光学顕微鏡および非線形光学顕微鏡法

磯部圭佑, 緑川克美
特開2011-145487 多光子励起顕微鏡

磯部圭佑, 緑川克美, 須田亮, 神成文彦, 宮脇敦史
特開2009-150649 空間情報検出装置

伊東一良, 磯部圭佑, 福井希一, 渡辺歴, 片岡省吾
特開2008-116409 屈折率測定方法および屈折率測定装置、光学部材の製造方法

伊東一良, 小関泰之, 磯部圭佑, 玉木隆幸, 片岡省吾, 川澄健人
特許第3885574号高調波干渉式分析方法及び装置

伊東一良, 福井希一, 渡邉歴, 磯部圭佑, 大久保邦彦
WO2006-104237 空間情報検出装置

伊東一良, 磯部圭佑, 福井希一, 渡邉歴, 片岡省吾
特開2006-010523 分子測定装置

大久保邦彦, 伊東一良, 福井希一, 磯部圭佑, 隅田剣生
特開2005-172776 複数の波長依存性物質を含む試料の分析方法及び分析装置

伊東一良, 福井希一, 渡邉歴, 磯部圭佑

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Research Interests

Research Areas

Research History

Awards

Papers

Misc.

Presentations

Industrial Property Rights

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