2005年
Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source
JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS
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- 巻
- 44
- 号
- 1-7
- 開始ページ
- L167
- 終了ページ
- L169
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1143/JJAP.44.L167
- 出版者・発行元
- INST PURE APPLIED PHYSICS
We report on a novel technique of multi-spectral two-photon-excited fluorescence microscopy by use of broadband supercontinuum light. A supercontinuum in the near-infrared region is generated in a 4.5-min-long photonic crystal fiber using a Ti:sapphire femtosecond oscillator. The supercontinuum is used as a multi-spectral excitation light source in a two-photon excited fluorescence microscope. We demonstrate that three-color fluorescence images of organelles in a cell can be simultaneously acquired.
- リンク情報
- ID情報
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- DOI : 10.1143/JJAP.44.L167
- ISSN : 0021-4922
- Web of Science ID : WOS:000227675600054