2015年8月
Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy
REVIEW OF SCIENTIFIC INSTRUMENTS
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- 巻
- 86
- 号
- 8
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1063/1.4927532
- 出版者・発行元
- AMER INST PHYSICS
We demonstrate and theoretically analyze the two-dimensional spatiotemporal focusing of femtosecond pulses by utilizing a two-dimensional spectral disperser. Compared with spatiotemporal focusing with a diffraction grating, it can achieve widefield illumination with better sectioning ability for a multiphoton excitation process. By utilizing paraxial approximation, our analytical method improves the axial confinement ability and identifies that the free spectra range (FSR) of the two-dimensional spectral disperser affects the out-of-focus multiphoton excitation intensity due to the temporal self-imaging effect. Based on our numerical simulation, a FSR of 50 GHz is necessary to reduce the out-of-focus two-photon excitation by 2 orders of magnitude compared with that in a grating-based spatiotemporal focusing scheme for a 90-fs excitation laser pulse. We build a two-dimensional spatiotemporal focusing microscope using a virtually imaged phased array and achieve an axial resolution of 1.3 mu m, which outperforms the resolution of conventional spatiotemporal focusing using a grating by a factor of 1.7, and demonstrate better image contrast inside a tissue-like phantom. (C) 2015 AIP Publishing LLC.
- リンク情報
- ID情報
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- DOI : 10.1063/1.4927532
- ISSN : 0034-6748
- eISSN : 1089-7623
- Web of Science ID : WOS:000360648800031