論文

査読有り 国際誌
2018年2月23日

Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the third isozyme with a distinct expression profile.

Bioscience, biotechnology, and biochemistry
  • Taiji Nomura
  • ,
  • Ryo Kuchida
  • ,
  • Naoki Kitaoka
  • ,
  • Yasuo Kato

82
5
開始ページ
1
終了ページ
11
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2018.1438170

6-Tuliposide B (PosB), a major secondary metabolite that accumulates in tulip (Tulipa gesneriana), is converted to the antibacterial lactone, tulipalin B (PaB), by PosB-converting enzyme (TCEB). TgTCEB1 and TgTCEB-R, which encode TCEB, are specifically expressed in tulip pollen and roots, respectively, but are hardly expressed in other tissues (e.g. leaves) despite the presence of substantial PosB-converting activity, suggesting the existence of another TCEB isozyme. Here, we describe the identification of TgTCEB-L ("L" for leaf), a paralog of TgTCEB1 and TgTCEB-R, from leaves via native enzyme purification. The enzymatic characters of TgTCEB-L, including catalytic activity and subcellular localization, were substantially the same as those of TgTCEB1 and TgTCEB-R. However, TgTCEB-L did not exhibit tissue-specific expression. Identification of TgTCEB-L explains the PosB-converting activity detected in tissues where TgTCEB1 and TgTCEB-R transcripts could not be detected, indicating that tulip subtilizes the three TgTCEB isozymes depending on the tissue.

リンク情報
DOI
https://doi.org/10.1080/09168451.2018.1438170
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29475400
ID情報
  • DOI : 10.1080/09168451.2018.1438170
  • PubMed ID : 29475400

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