論文

査読有り 国際誌
2019年3月2日

Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing.

International journal of molecular sciences
  • Sopak Supakul
  • ,
  • Kenta Yao
  • ,
  • Hiroki Ochi
  • ,
  • Tomohito Shimada
  • ,
  • Kyoko Hashimoto
  • ,
  • Satoko Sunamura
  • ,
  • Yo Mabuchi
  • ,
  • Miwa Tanaka
  • ,
  • Chihiro Akazawa
  • ,
  • Takuro Nakamura
  • ,
  • Atsushi Okawa
  • ,
  • Shu Takeda
  • ,
  • Shingo Sato

20
5
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms20051079

Pericytes are mesenchymal cells that surround the endothelial cells of small vessels in various organs. These cells express several markers, such as NG2, CD146, and PDGFRβ, and play an important role in the stabilization and maturation of blood vessels. It was also recently revealed that like mesenchymal stem cells (MSCs), pericytes possess multilineage differentiation capacity, especially myogenic, adipogenic, and fibrogenic differentiation capacities. Although some previous studies have reported that pericytes also have osteogenic potential, the osteogenesis of pericytes can still be further elucidated. In the present study, we established novel methods for isolating and culturing primary murine pericytes. An immortalized pericyte line was also established. Multilineage induction of the pericyte line induced osteogenesis, adipogenesis, and chondrogenesis of the cells in vitro. In addition, pericytes that were injected into the fracture site of a bone fracture mouse model contributed to callus formation. Furthermore, in vivo pericyte-lineage-tracing studies demonstrated that endogenous pericytes also differentiate into osteoblasts and osteocytes and contribute to bone fracture healing as a cellular source of osteogenic cells. Pericytes can be a promising therapeutic candidate for treating bone fractures with a delayed union or nonunion as well as bone diseases causing bone defects.

リンク情報
DOI
https://doi.org/10.3390/ijms20051079
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30832329
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429337

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