Mar, 2013
A tilting embryo culture system increases the number of high-grade human blastocysts with high implantation competence
REPRODUCTIVE BIOMEDICINE ONLINE
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- Volume
- 26
- Number
- 3
- First page
- 260
- Last page
- 268
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.rbmo.2012.11.014
- Publisher
- ELSEVIER SCI LTD
Human embryos normally experience mechanical stimuli during development in vivo. To apply appropriate stimuli to embryos, this study group developed a tilting embryo culture system (TECS) and investigated whether it could improve the grade of fresh human embryos compared with a control static culture system. A total of 450 retrieved oocytes from 32 IVF or intracytoplasmic sperm injection cycles of 32 women were cultured for 5-6 days. Oocytes were divided randomly into TECS and control groups and then were inseminated in vitro. All embryos were evaluated at days 3 and 5 using standard grading criteria for embryo quality. The rates of fertilization per mature oocyte and high-grade cleavage-stage embryo formation in the TECS group were similar to those in the control group. The rates of blastocyst formation and of blastocysts graded 3BB or higher at day 5 were significantly higher in the TECS group than those in the control group: 45.3% (67/148) versus 32.1% (51/159) (P = 0.018) and 29.1% (43/148) versus 17.6% (28/159) (P = 0.018), respectively. The TECS group produced more high-grade blastocysts than the control group. Embryo movement or mechanical stimulation during embryo culture may be beneficial for human embryonic development. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
- Link information
- ID information
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- DOI : 10.1016/j.rbmo.2012.11.014
- ISSN : 1472-6483
- Web of Science ID : WOS:000315709600011