論文

国際誌
2023年3月28日

Two-point optical manipulation reveals mechanosensitive remodeling of cell-cell contacts in vivo.

Proceedings of the National Academy of Sciences of the United States of America
  • Kenji Nishizawa
  • ,
  • Shao-Zhen Lin
  • ,
  • Claire Chardès
  • ,
  • Jean-François Rupprecht
  • ,
  • Pierre-François Lenne

120
13
開始ページ
e2212389120
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.2212389120

Biological tissues acquire reproducible shapes during development through dynamic cell behaviors. Most of these behaviors involve the remodeling of cell-cell contacts. During epithelial morphogenesis, contractile actomyosin networks remodel cell-cell contacts by shrinking and extending junctions between lateral cell surfaces. However, actomyosin networks not only generate mechanical stresses but also respond to them, confounding our understanding of how mechanical stresses remodel cell-cell contacts. Here, we develop a two-point optical manipulation method to impose different stress patterns on cell-cell contacts in the early epithelium of the Drosophila embryo. The technique allows us to produce junction extension and shrinkage through different push and pull manipulations at the edges of junctions. We use these observations to expand classical vertex-based models of tissue mechanics, incorporating negative and positive mechanosensitive feedback depending on the type of remodeling. In particular, we show that Myosin-II activity responds to junction strain rate and facilitates full junction shrinkage. Altogether our work provides insight into how stress produces efficient deformation of cell-cell contacts in vivo and identifies unanticipated mechanosensitive features of their remodeling.

リンク情報
DOI
https://doi.org/10.1073/pnas.2212389120
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/36947511
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10068846
ID情報
  • DOI : 10.1073/pnas.2212389120
  • PubMed ID : 36947511
  • PubMed Central 記事ID : PMC10068846

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