2014年
Rapid depletion of budding yeast proteins via the fusion of an auxin-inducible degron (AID)
Current Protocols in Cell Biology
- ,
- 巻
- 2014
- 号
- 開始ページ
- 20.9.1
- 終了ページ
- 20.9.16
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/0471143030.cb2009s64
- 出版者・発行元
- John Wiley and Sons Inc.
The auxin-inducible degron (AID) system allows the rapid and reversible proteolysis of proteins of interest, and enables the generation of conditional mutants of budding yeast. The construction of budding yeast AID mutants is simple, and the effect of depletion of essential proteins on proliferation can be confirmed by analyzing their phenotype. In this protocol, we describe a procedure to generate AID mutants of budding yeast via a simple transformation using PCR-amplified DNA. We also describe methods to confirm the depletion of proteins of interest that are required for proliferation by serial-dilution and liquid-culture assays.
- リンク情報
- ID情報
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- DOI : 10.1002/0471143030.cb2009s64
- ISSN : 1934-2616
- ISSN : 1934-2500
- PubMed ID : 25181302
- SCOPUS ID : 84949201120