2013年4月
The Elg1 Replication Factor C-like Complex Functions in PCNA Unloading during DNA Replication
MOLECULAR CELL
- ,
- ,
- ,
- 巻
- 50
- 号
- 2
- 開始ページ
- 273
- 終了ページ
- 280
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.molcel.2013.02.012
- 出版者・発行元
- CELL PRESS
The ring-shaped complex PCNA coordinates DNA replication, encircling DNA to act as a polymerase clamp and a sliding platform to recruit other replication proteins. PCNA is loaded onto DNA by replication factor C, but it has been unknown how PCNA is removed from DNA when Okazaki fragments are completed or the replication fork terminates. Here we show that the Elg1 replication factor C-like complex (Elg1-RLC) functions in PCNA unloading. Using an improved degron system we show that without Elg1, PCNA accumulates on Saccharomyces cerevisiae chromatin during replication. The accumulated PCNA can be removed from chromatin in vivo by switching on Elg1 expression. We find moreover that treating chromatin with purified Elg1-RLC causes PCNA unloading in vitro. Our results demonstrate that Elg1-RLC functions in unloading of both unmodified and SUMOylated PCNA during DNA replication, while the genome instability of an elg1 Delta mutant suggests timely PCNA unloading is critical for chromosome maintenance.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.molcel.2013.02.012
- ISSN : 1097-2765
- PubMed ID : 23499004
- Web of Science ID : WOS:000318208600012