Papers

Peer-reviewed
Mar 1, 2018

The Ferret as a Surgical Model for Vocal Fold Scar Creation and Treatment

Annals of Otology, Rhinology and Laryngology
  • Haruka Kodama
  • ,
  • Yoshihiko Kumai
  • ,
  • Kohei Nishimoto
  • ,
  • Yutaka Toya
  • ,
  • Satoru Miyamaru
  • ,
  • Shinobu Furushima
  • ,
  • Eiji Yumoto

Volume
127
Number
3
First page
146
Last page
154
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1177/0003489417750165
Publisher
SAGE Publications Inc.

Objectives: To develop a vocal fold (VF) scarring procedure in the ferret, characterize the scars histologically, and test the injectability of the lamina propria (LP). Secondarily, to compare laryngeal anatomy of the ferret with rat and rabbit. Materials and Methods: The larynges of 18 male ferrets were prepared by unilateral scarring, and normal larynges from 6 female Wistar rats and 5 male albino rabbits were used for comparative purposes. For scarring, the right VF were electrocauterized, ablating the entire LP. Prior to harvesting the larynges at 4 and 16 weeks, each ferret was re-anesthetized, and in 3 animals, India ink was injected into the LPs of both normal and scarred VFs. Results: Laryngoscopic methods and instrumentation for precise visualization, scarring, and injection were developed. The scarred VFs had reduced hyaluronic acid and increased collagen type I, III, and fibronectin compared with normal VFs. The 2 timepoints (4 and 16 weeks) differed significantly only in collagen type III level (levels were higher at 4 weeks). Injected ink migrated from scarred LP to muscle layer just beneath the scarred tissue 3 hours after injection. Conclusion: The ferret is a promising species for creation and experimental treatment of vocal fold scar.

Link information
DOI
https://doi.org/10.1177/0003489417750165
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29310440
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042281273&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85042281273&origin=inward
ID information
  • DOI : 10.1177/0003489417750165
  • ISSN : 0003-4894
  • Pubmed ID : 29310440
  • SCOPUS ID : 85042281273

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