MISC

2002年6月

Distribution of dynamins in testis and their possible relation to spermatogenesis

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • A Kamitani
  • ,
  • H Yamada
  • ,
  • M Kinuta
  • ,
  • M Watanabe
  • ,
  • SA Li
  • ,
  • T Matsukawa
  • ,
  • M McNiven
  • ,
  • H Kumon
  • ,
  • K Takei

294
2
開始ページ
261
終了ページ
267
記述言語
英語
掲載種別
DOI
10.1016/S0006-291X(02)00470-9
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Dynamin 2 and dynamin 3 are highly expressed in testis. However, their functions in the tissue remain unclear. Considering that dynamin 1, neuron-specific isoform of dynamin, plays a pivotal role in endocytosis, functions of dynamin 2 and dynamin 3 in testis must be essential. Cellular expression and subcellular localization of dynamin 2 and dynamin 3 in testis were investigated. Dynamin 2 and dynainin 3 were highly expressed in germ cells and Sertoli cells, constituents of seminiferous tubules. By immunofluorescence it was revealed that dynamin 2 colocalizes with clathrin both at the plasmamembrane and at Golgi in a cell line of Sertoli cells. Immuno reactivity for dynamin 3, on the other hand, appeared as finer puncta, which did not colocalize with clathrin, suggesting that these two dynamins have distinct functions in Sertoli cells. In the klotho deficient mouse testis, which demonstrates disorder in spermatogenesis, expression of dynamin 2 and dynamin 3 was drastically reduced indicating possible association of these proteins with spermatogenesis. (C) 2002 Elsevier Science (USA). All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/S0006-291X(02)00470-9
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000176340800012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/S0006-291X(02)00470-9
  • ISSN : 0006-291X
  • Web of Science ID : WOS:000176340800012

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